Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium for culturing Marc-145 cell and preparation method of culture medium

A culture medium and cell technology, applied in biochemical equipment and methods, culture process, tissue culture, etc., can solve the problems of high price of fetal bovine serum, increased burden on manufacturers and consumers, and higher vaccine production costs, etc., to achieve Effects of large-scale production, lower production cost, and reduced usage

Active Publication Date: 2015-05-20
ZHAOQING DAHUANONG BIOLOGIC PHARMA +1
View PDF5 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The price of fetal bovine serum is relatively expensive, and a large amount needs to be added to the Marc-145 cell culture medium, which increases the cost of vaccine production and increases the burden on manufacturers and consumers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium for culturing Marc-145 cell and preparation method of culture medium
  • Culture medium for culturing Marc-145 cell and preparation method of culture medium
  • Culture medium for culturing Marc-145 cell and preparation method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A medium for cultivating Marc-145 cells, which is composed of the following components: DMEM, enzymatic gelatin solution, amino acid DMEM solution and fetal bovine serum; the DMEM, enzymatic gelatin solution, amino acid DMEM solution and fetal bovine serum The volume ratio is: enzymolysis gelatin solution: amino acid DMEM solution: fetal bovine serum: DMEM=1:2:X:100;

[0028] The enzymatic gelatin solution is trypsin enzymatic gelatin solution, and the PBS solution is prepared with ultrapure water; the protein concentration in the enzymatic gelatin solution is 50mg / ml, and the solvent in the enzymatic gelatin solution For PBS solution;

[0029] The solvent in the amino acid DMEM solution is DMEM, and the solute is composed of the following components according to the mass volume ratio: alanine 5mg / L, arginine 10mg / L, asparagine 5mg / L, aspartic acid 15mg / L, cystine 5mg / L, glutamic acid 50mg / L, glutamine 40mg / L, glycine 5mg / L, histidine 5mg / L, isoleucine 5mg / L, leucine ...

Embodiment 2

[0043] A medium for cultivating Marc-145 cells, which is composed of the following components: DMEM, enzymatic gelatin solution, amino acid DMEM solution and fetal bovine serum; the DMEM, enzymatic gelatin solution, amino acid DMEM solution and fetal bovine serum The volume ratio is: enzymatic gelatin solution: amino acid DMEM solution: fetal bovine serum: DMEM=1:2:Y:100; the enzymatic gelatin solution is trypsin enzymatic gelatin solution, and the PBS solution is prepared with ultrapure water form; the protein concentration in the enzymatic gelatin solution is 70mg / ml, and the solvent in the enzymatic gelatin solution is PBS solution;

[0044] The solvent in the amino acid DMEM solution is DMEM, and the solute is composed of the following components according to the mass volume ratio: alanine 8mg / L, arginine 12mg / L, asparagine 9mg / L, aspartic acid 20mg / L, cystine 7mg / L, glutamic acid 60mg / L, glutamine 50mg / L, glycine 10mg / L, histidine 8mg / L, isoleucine 8mg / L, leucine 12mg / L ...

Embodiment 3

[0057] A medium for cultivating Marc-145 cells, which is composed of the following components: DMEM, enzymatic gelatin solution, amino acid DMEM solution and fetal bovine serum; the DMEM, enzymatic gelatin solution, amino acid DMEM solution and fetal bovine serum The volume ratio is: enzymatic gelatin solution: amino acid DMEM solution: fetal bovine serum: DMEM=2:5:Z:100; the protein concentration in the enzymatic gelatin solution is 20 mg / ml, and the protein concentration in the enzymatic gelatin solution The solvent is PBS solution; the enzymolysis gelatin solution is trypsin enzymolysis gelatin solution, and the PBS solution is prepared with ultrapure water;

[0058] The solvent in the amino acid DMEM solution is DMEM, and the solute is composed of the following components according to the mass volume ratio: alanine 35mg / L, arginine 60mg / L, asparagine 25mg / L, aspartic acid 80mg / L, cystine 30mg / L, glutamic acid 100mg / L, glutamine 80mg / L, glycine 20mg / L, histidine 10mg / L, is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a culture medium for culturing a Marc-145 cell. The culture medium consists of the following components: DMEM (dulbecco's modified eagle medium), an enzymolysis gelatin solution, amino acid DMEM solution and fetal calf serum in a volume ratio of (1-2):(1-5):(2-5):100. The invention further provides a preparation method of the culture medium, wherein the preparation method comprises the following steps: respectively weighing the DMEM, the enzymolysis gelatin solution, the amino acid DMEM solution and the fetal calf serum according to the volume ratio of a formula, and mixing to obtain the culture medium. The culture medium for culturing the Marc-145 cell disclosed by the invention can greatly reduce usage amount of the bovine serum in a Marc-145 cell culture process, so that the production cost is reduced; meanwhile, the preparation method is simple and easy to operate.

Description

technical field [0001] The invention relates to a medium and a preparation method thereof, in particular to a medium for cultivating Marc-145 cells and a preparation method thereof. Background technique [0002] Pig PRRS, also known as "Porcine Reproductive and Respiratory Disorder Syndrome", is caused by the Porcine Reproductive and Respiratory Disorder Syndrome Virus. It is an immunosuppressive disease, often secondary to other pathogenic infections. Attenuated vaccines or inactivated vaccines are used to prevent highly pathogenic PRRS in my country. Marc-145 cells need to be cultivated in the production process of inactivated vaccine or live vaccine of highly pathogenic PRRS virus. [0003] In the existing Marc-145 cell culture process, it is usually necessary to add 8-10% bovine serum for culturing, and if less bovine serum is added, the growth and reproduction speed of Marc-145 cells will be too slow. For example, the invention patent application document with public...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0686C12N2500/30C12N2500/32C12N2500/84
Inventor 徐家华陈瑞爱蒋春英张东霞王新秋
Owner ZHAOQING DAHUANONG BIOLOGIC PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com