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Extraction method of SOD in emblic leafflower fruits

An extraction method and technology of emblica, applied in the direction of biochemical equipment and methods, enzymes, oxidoreductases, etc., can solve the problems of extraction process research, undiscovered reports of SOD extraction, etc., and achieve easy-to-obtain raw materials, simple equipment and processes , the effect of high purity

Inactive Publication Date: 2015-05-20
THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Amla contains active substance SOD that is beneficial to human body, but so far there is no in-depth study on its extraction process, and no report about extracting SOD from Amla

Method used

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Examples

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Embodiment 1

[0021] In an embodiment of the present invention, a method for extracting SOD of Amla emblica, the specific steps include:

[0022] (1) Cleaning, pitting, and squeezing: washing and pitting the fresh Emlical emblica, putting the pulp into a squeeze machine to squeeze, and obtaining squeezed fruit juice;

[0023] (2) Heating: heating the fruit juice squeezed in step (1), the heating temperature is 85° C., and the heating time is 1 hour to obtain denatured fruit juice;

[0024] (3) centrifugation: after the fruit juice denatured in step (2) is down to normal temperature, carry out centrifugation, centrifugal speed is 6000r / min, and centrifugation time is 35min, filters, and obtains SOD crude enzyme liquid;

[0025] (4) in the SOD thick enzyme liquid of step (3), add phosphate buffer saline and quartz sand and carry out centrifugal separation, the pH of phosphate buffer saline is 7.0, and the add-on of phosphate buffer saline is SOD thick enzyme liquid 100% of the mass, the addi...

Embodiment 2

[0030] In an embodiment of the present invention, a method for extracting SOD of Amla emblica, the specific steps include:

[0031] (1) Cleaning, pitting, and squeezing: washing and pitting the fresh Emlical emblica, putting the pulp into a squeeze machine to squeeze, and obtaining squeezed fruit juice;

[0032] (2) Heating: heating the fruit juice squeezed in step (1), the heating temperature is 90°C, and the heating time is 1.2h, to obtain denatured fruit juice;

[0033] (3) centrifugation: after the fruit juice denatured in step (2) is down to normal temperature, carry out centrifugation, centrifugal speed is 7500r / min, centrifugation time is 30min, filters, obtains SOD crude enzyme liquid;

[0034] (4) in the SOD crude enzyme liquid of step (3), add phosphate buffer and quartz sand and carry out centrifugation, the pH of phosphate buffer is 7.2, and the add-on of phosphate buffer is the SOD crude enzyme liquid quality 110%, the addition of quartz sand is 3% of the mass of...

Embodiment 3

[0039] In an embodiment of the present invention, a method for extracting SOD of Amla emblica, the specific steps include:

[0040] (1) Cleaning, pitting, and squeezing: washing and pitting the fresh Emlical emblica, putting the pulp into a squeeze machine to squeeze, and obtaining squeezed fruit juice;

[0041] (2) Heating: heating the fruit juice squeezed in step (1), the heating temperature is 95°C, and the heating time is 1.5h to obtain denatured fruit juice;

[0042] (3) centrifugation: after the juice denatured in step (2) is down to normal temperature, carry out centrifugation, centrifugation speed is 9000r / min, centrifugation time is 25min, filters, obtains SOD crude enzyme liquid;

[0043] (4) in the SOD thick enzyme liquid of step (3), add phosphate buffer and quartz sand and carry out centrifugation, the pH of phosphate buffer is 7.4, and the add-on of phosphate buffer is the quality of SOD thick enzyme liquid 120%, the addition of quartz sand is 5% of the mass of ...

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Abstract

The invention discloses an extraction method of SOD in emblic leafflower fruits. The extraction method of SOD in the emblic leafflower fruits comprises the following steps: washing, denucleating, squeezing, heating and centrifugally separating fresh emblic leafflower fruits to prepare an SOD crude enzyme solution; adding a phosphate buffer solution and quartz sand into the SOD crude enzyme solution and centrifugally separating to prepare an SOD clear filtrate; and carrying out chromatographic separation, vacuum concentration, dialysis and vacuum drying on the SOD clear filtrate to prepare SOD. The extraction method of SOD in the emblic leafflower fruits is simple in equipment and process, easily available in raw materials and low in cost; by virtue of the adoption of the phosphate buffer solution, the separation efficiency is improved; meanwhile, the activity of SOD is well protected; the quality of the prepared SOD is improved; and the prepared SOD is high in purity.

Description

technical field [0001] The invention relates to an enzyme extraction method, in particular to an extraction method of SOD of Amla emblica. Background technique [0002] Superoxide dismutase (SOD) can resist and block the damage to cells caused by oxygen free radicals, repair damaged cells in time, and restore the damage to cells caused by free radicals. Due to the pressure of modern life, environmental pollution, various radiation and excessive exercise will cause a large number of oxygen free radicals to form; therefore, the position of SOD in the biological antioxidant mechanism is becoming more and more important. [0003] Amla contains active substance SOD which is beneficial to the human body, but so far there is no in-depth study on its extraction process, and no report about extracting SOD from Amla. Contents of the invention [0004] The object of the present invention is to provide a low-cost, high-purity extraction method of Emlical emblica SOD, to solve the pro...

Claims

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Application Information

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IPC IPC(8): C12N9/02
CPCC12N9/0089C12Y115/01001
Inventor 冯颖张宏侯彬赵宇
Owner THE RES INST OF RESOURCES INSECTS RIRI OF THE CHINESE ACADEMY OF FORESTRY
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