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Method for preparing microbial oil by utilizing wood hydrolysate

A technology of microbial oil and hydrolyzate, applied in the field of microbial fermentation, can solve the problems of high cost and achieve the effects of short production cycle, simple operation and rich forest resources

Inactive Publication Date: 2015-05-20
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the technical problem of high cost of carbon source raw materials for the production of existing microbial oils, and provide a low-cost raw material for the production of microbial oils that can be applied to biodiesel

Method used

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  • Method for preparing microbial oil by utilizing wood hydrolysate
  • Method for preparing microbial oil by utilizing wood hydrolysate
  • Method for preparing microbial oil by utilizing wood hydrolysate

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present invention utilizes wood hydrolyzate to prepare the method for microbial grease, adopts wood as coniferous material, is specifically pine wood; The oleaginous bacterium that adopts is rhodococcus opacus ( Rhodococcus opacus ) PD 630, the strain number is DSM 44193.

[0034] Specifically include the following steps:

[0035] (1) Hot water treatment: Slicing and crushing pine wood to obtain pine wood powder. Weigh 100g of pine wood powder, add 800 ml of deionized water, mix evenly, put it into the reaction kettle, and react at 180°C for 1 h (reaction at 150-200°C for 30-120 min can also be implemented), Take out and filter, collect filtrate to obtain wood hydrolyzate.

[0036] (2) Detoxification:

[0037]2.1. Add Ca(OH) to the wood hydrolyzate prepared in step (1) 2 , so that the pH is 10, after 1 h, filter the hydrolyzate and take the filtrate to obtain an alkaline wood hydrolyzate; add 2 M H to the alkaline wood hydrolyzate 2 SO 4 , adjust the pH to 5.5...

Embodiment 2

[0048] The present invention utilizes wood hydrolyzate to prepare the method for microbial grease, adopts wood as coniferous material, is specifically pine wood; The oleaginous bacterium that adopts is Rhodococcus opacus ( Rhodococcus opacus ), the strain number is DSM 1069.

[0049] (1) Hot water treatment: Slicing and crushing pine wood to obtain pine wood powder. Weigh 150g of pine wood powder, add 800 ml of deionized water, mix evenly, put it into the reaction kettle, and react at 200°C for 1 h (reaction at 150-200°C for 30-120 min can also be implemented), Take out and filter, collect filtrate to obtain wood hydrolyzate.

[0050] (2) Detoxification:

[0051] 2.1. Add Ca(OH) to the wood hydrolyzate prepared in step (1) 2， Make the pH to 11, filter the hydrolyzate after 1 h and take the filtrate to obtain the alkaline wood hydrolyzate; add 2 M H to the alkaline wood hydrolyzate 2 SO 4 , adjust the pH to 5, filter again after 1 h to obtain the acidic wood hydrolyzate; a...

Embodiment 3

[0062] The present invention utilizes wood hydrolyzate to prepare the method for microbial grease, adopts wood as coniferous material, is specifically sweetgum wood; The oleaginous bacterium that adopts is rhodococcus opacus ( Rhodococcus opacus ) PD 630, the strain number is DSM 44193.

[0063] (1) Hot water treatment: slice sweetgum wood and crush it to obtain pine wood powder. Weigh 120g of pine wood powder, add 600 ml of deionized water, mix evenly, put it into the reaction kettle, and react at 200°C for 1 h (reaction at 150-200°C for 30-120 min can also be implemented), Take out and filter, collect filtrate to obtain wood hydrolyzate.

[0064] (2) Detoxification:

[0065] 2.1. Add Ca(OH) to the wood hydrolyzate prepared in step (1) 2， Make the pH to 12, after 1 h, filter the hydrolyzate and take the filtrate to obtain an alkaline wood hydrolyzate; add 2 M H to the alkaline wood hydrolyzate 2 SO 4 , adjust the pH to 6, filter again after 1 h to obtain the acidic wood ...

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Abstract

The invention discloses a method for preparing microbial oil by utilizing wood hydrolysate. The method comprises the following steps: performing hot water treatment on woods, thereby obtaining wood hydrolysate; expelling toxins of the wood hydrolysate, adding salt into the detoxicated wood hydrolysate, regulating the pH value to be 7-9, sterilizing, thereby obtaining a culture medium; inoculating a seed solution producing oil bacteria into the culture medium, fermenting for 32-56 hours, thereby obtaining fermentation liquor; and collecting the thalli in the fermentation liquor, carrying out an oil exchange reaction, thereby obtaining the microbial oil. The microbial oil provided by the invention is simple in production process and low in operating cost, a novel path of converting lignocellulose raw materials into biological energy source is provided, and resource waste and environmental pollution brought by emission of lignocellulose raw materials can be effectively reduced.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a method for preparing microbial oil by using wood hydrolyzate. Background technique [0002] The massive consumption of traditional energy materials such as coal, oil and natural gas makes it increasingly urgent to find alternative renewable energy sources. Biomass energy is the form of energy in which solar energy is stored in biomass in the form of chemical energy. It plays an important role in the entire energy system. It is directly or indirectly derived from the photosynthesis of green plants and is a renewable green energy source. Woody biomass is an important part of biomass. The total amount of terrestrial forest biomass resources in China is more than 18 billion tons, but direct combustion has been the main way of utilizing woody biomass energy in my country for a long time. In recent years, the research and application of woody biomass energy has developed rapid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/01
CPCC12P7/6463C12P2203/00
Inventor 危臻晏铭曾光明黄丹莲赖萃黄超许飘李宁杰程敏张辰
Owner HUNAN UNIV