Nonlinear correction method of cell DNA detection equipment

Abstract

A nonlinear correction method of cell DNA detection equipment comprises the following steps: setting an index model transformation adjusting parameter, a Bayer mode analysis adjusting parameter, and an image segmentation threshold compensation parameter; adjusting the microscope in cell DNA detection equipment, placing a standard pig liver correction slice, setting a scanning zone, then starting to scan; obtaining a scan image after scanning, obtaining quality control parameters through system calculation on the scan image, comparing the obtained quality control parameters with standard quality control parameters in the system, adjusting the index model transformation adjusting parameter and Bayer mode analysis adjusting parameter according to the deviation; and repeating the steps mentioned above until the deviation between the calculate quality control parameters and corresponding standard quality control parameters in the system falls in an allowable error range so as to complete the correction. The detection equipment is corrected and adjusted by adjusting several parameters of a nonlinear mode and adopting a limited iterative method, and the corrected equipment can be applied to normal work quickly.

Description

Technical field [0001] The invention relates to the field of medical testing equipment, in particular to a calibration method of cell DNA testing equipment. Background technique [0002] Changes in the DNA content in the cell nucleus are often an early specific indication of the onset of tumors. Through the quantitative detection of the DNA content in the cell nucleus, early lesions can be detected in time. The automatic quantitative analysis system of cellular DNA images based on Forggen staining realizes early diagnosis of cancer and pre-cancer lesions by quantitatively detecting changes in DNA content in the nucleus. [0003] At present, there is no simple and effective method to calibrate various improved and changed hardware components in various detection equipment for automatic scanning and analysis of cell DNA. For example, the components in the detection equipment that affect the calculation of cell DNA include stains for cell slices, Dyeing process, light source, con...

AI Technical Summary

Problems solved by technology

[0003] Currently, there is no simple and effective way to correct various improved and changed hardware components in the current detection equipment for automatic scanning and analysis of cell DNA. Optical components and optical processing components such as dyeing process, light source, condenser lens, filter, objective lens, optical connector, infrared filter of imaging equipment, Bayer mode of color imaging chip and interpolation analysis

The main reason is that the characteristics of each optical component in the optical path of the system are complex, and the change of light presents a nonlinear change, which makes the linear background compensation deviation in the conventional optical density calculation, resulting in a system hardware configuration. The method and process of the developed cell DNA quantitative analysis and detection equipment may fail in the new hardware environment, and need to be re-developed, which is time-consuming and labor-intensive

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