Electrochemical immunosensing method for detecting Listeria monocytogenes

Abstract

The invention discloses an electrochemical immunosensing method for detecting Listeria monocytogenes, belonging to the field of the biotechnological detection. The surface of a gold electrode is activated electrochemically and then is immersed in 1% of 3-mercaptopropionic acid ethanol solution to be self-assembled to form a monomolecular film, a capture antibody is combined to the surface of the electrode after activation, an enzyme amplification signal is introduced by a sandwich method, and an enzymatic electroactive substance is subjected to oxidation reduction to obtain a current response signal, therefore, a method for detecting the Listeria monocytogenes by use of the electrochemical immunosensor adopting three electrodes as a system is established; the method has good sensitivity, specificity and stability; the detection system is small, the reagent is saved, the detection cost is reduced remarkably, the method has great potential in application and practical sample detection due to no influence from the turbidity of the sample.

Description

technical field [0001] The invention relates to a method for detecting Listeria monocytogenes by a chronoamperometry based on sandwich method electrochemical immunosensing, and belongs to the technical field of biotechnology detection. Background technique [0002] Listeria monocytogenes is an important pathogen of zoonotic and food-borne diseases. It can cause listeriosis in humans and animals. After infection, the main manifestations are meningitis, sepsis, abortion, etc., and the fatality rate is as high as 30% to 70%, this bacterium has been listed as one of the four major food pathogens in the 1990s in the world. FDA, WHO and other organizations have successively established corresponding institutions or stipulated corresponding strategies to prevent and control the contamination and spread of the bacteria. In 1988, WHO issued an advisory book on "Foodborne Listeriosis", guiding countries around the world on how to prevent LM pollution and poisoning, and advising publi...

AI Technical Summary

Problems solved by technology

Among these methods, the results of conventional culture methods are accurate and reliable, but time-consuming and laborious, and it usually takes 5-7 days to obtain the results; the ELISA method is simple to operate, has strong specificity, and high reaction sensitivity, but it needs pretreatment or enrichment of samples before analysis. set; PCR analysis requires DNA extraction and amplification, etc., the steps are cumbersome and prone to false positives

In summary, these methods require well-trained professionals and are expensive and long analysis times

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