Tissue culture and rapid propagation method for dianthus caryophyllus L

A technology of carnation group and proliferation medium, which is applied in the field of plant tissue culture, can solve the problems of accumulating viruses, reducing the yield and quality of carnation, and achieves the effects of maintaining excellent characters, promoting large-scale production and overcoming viruses.

Inactive Publication Date: 2015-06-03
黎有辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Carnation seedlings are usually propagated by traditional cutting methods. As the number of reproduction generations increases, viruses tend to accumulate in the plants, thereby reducing the yield and quality of Carnation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Induction culture: take the vigorously growing lateral buds, remove the leaves, disinfect with 75% alcohol on the ultra-clean workbench for 30 seconds, then disinfect with 0.1% mercury chloride solution for 8 minutes, and finally rinse with sterile water for 3 times. After disinfection, under a binocular dissecting microscope, use a scalpel to completely peel off the base of the outer young leaf stem, so that the shoot tip is fully exposed, and cut off the shoot tip, with a length of 0.3-0.5mm and 2-4 leaf primordia , Immediately after cutting, they were inoculated in induction medium for adventitious bud induction. After inoculation, it was placed in the light for 24 hours a day, the light intensity was 1000lx, the culture temperature was 25°C, and the relative air humidity was 75%. After 14 days of cultivation, the contamination rate was 3.9%, and after 30 days of cultivation, the induction rate was 95.38%. The induction medium is: modified MS (Mg and Fe doubled)+...

Embodiment 2

[0021] (1) Induction culture: take the vigorously growing lateral buds, remove the leaves, disinfect with 75% alcohol on the ultra-clean workbench for 45 seconds, then disinfect with 0.1% mercury chloride solution for 10 minutes, and finally rinse with sterile water for 5 times. After disinfection, under a binocular dissecting microscope, use a scalpel to completely peel off the base of the outer young leaf stem, so that the shoot tip is fully exposed, and cut off the shoot tip, with a length of 0.3-0.5mm and 2-4 leaf primordia , Immediately after cutting, they were inoculated in induction medium for adventitious bud induction. After inoculation, it was placed in the light for 24 hours a day, the light intensity was 1500lx, the culture temperature was 26°C, and the relative air humidity was 75%. After 14 days of cultivation, the contamination rate was 13.9%, and after 30 days of cultivation, the induction rate was 91.93%. The induction medium is: modified MS (Mg and Fe doubled...

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PUM

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Abstract

The invention discloses a tissue culture and rapid propagation method for dianthus caryophyllus L. The dianthus caryophyllus L belongs to caryophyllaceae dianthus herbaceous perennial flowers, has the advantages of high productbility, good ornamental value, easiness in storage and transportation, and the like, and is one of main culture varieties of commercial cut-flowers cultured most widely in the present world. A large number of high-quality seedlings can be obtained by plant in-vitro culture, the propagation coefficient is greatly increased, viruses carried in a plant body are reduced, the stress resistance of the whole plant is improved, the limitations of environmental conditions of seasons are avoided, and the defects of conventional cutting propagation are made up through the breeding mode. A lateral bud of dianthus caryophyllus L is taken as an explant, and a dianthus caryophyllus L in-vitro replanting plant is successfully obtained by adventive bud induction culture, multiplication culture, rooting culture, acclimatization and transplantation, and the like, a dianthus caryophyllus L tissue culture and rapid propagation system is established, and the large-scale production of dianthus caryophyllus L tissue culture seedlings is promoted.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of carnation. Background technique [0002] Carnation ( Dianthus caryopHyllus L ), also known as carnation, is a perennial herbaceous flower of the genus Dianthus in the Caryophyllaceae family. With its high productivity, good ornamental value, storage and transportation resistance, etc., it has become one of the main varieties of commercial cut flowers cultivated in the world today. In recent years, carnation has been loved by consumers for its unique characteristics, and is widely used in various gift decorations and high-end flowers. In 1910, my country introduced and cultivated Carnation from Holland. After long-term development, my country has become one of the major producers of carnation cut flowers. At present, Shanghai and Yunnan have become the breeding centers of carnation seedlings i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 黎有辉
Owner 黎有辉
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