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Vaccine adjuvant and application thereof in preparation of Newcastle disease inactivating vaccine

An inactivated vaccine and Newcastle disease technology, which is applied in the field of preparation of Newcastle disease inactivated vaccine, can solve the problems of unsatisfactory immune effect of the Newcastle disease inactivated vaccine, and achieve the effect of good protection, good safety and prolonging the protection period.

Active Publication Date: 2015-06-03
JILIN ZHENGYE BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the immune effect of inactivated Newcastle disease vaccine is not ideal, and it is urgent to develop an inactivated Newcastle disease vaccine that can stimulate the body to produce strong cellular immunity and humoral immunity

Method used

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  • Vaccine adjuvant and application thereof in preparation of Newcastle disease inactivating vaccine
  • Vaccine adjuvant and application thereof in preparation of Newcastle disease inactivating vaccine
  • Vaccine adjuvant and application thereof in preparation of Newcastle disease inactivating vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 aqueous phase solution preparation

[0026] 1, the preparation of levamisole mother liquor

[0027] Commercially available veterinary levamisole hydrochloride injection with a levamisole content of 50 mg / ml was used as the levamisole mother solution; it was filtered and sterilized with a 0.22 μm filter (purchased from Sartorius) before use.

[0028] 2. Preparation of aqueous solution

[0029] Get 4ml of 50mg / ml levamisole mother solution and mix with 96ml chicken Newcastle disease inactivated virus liquid, and adjust the pH value to reach neutrality, be the aqueous phase solution that contains levamisole in every milliliter of solution and be 2mg.

Embodiment 2

[0030] Embodiment 2 water phase solution preparation

[0031] 1, the preparation of levamisole mother liquor

[0032] Commercially available veterinary levamisole hydrochloride injection with a levamisole content of 50 mg / ml was used as the levamisole mother solution; it was filtered and sterilized with a 0.22 μm filter (purchased from Sartorius) before use.

[0033] 2. Preparation of aqueous solution

[0034] Get 10ml of 50mg / ml levamisole mother solution and 90ml chicken Newcastle disease inactivated virus solution and mix evenly, and adjust the pH value to reach neutrality, namely be the aqueous phase solution that contains levamisole in every milliliter of solution and be 5mg.

Embodiment 3

[0035] Embodiment 3 aqueous phase solution preparation

[0036] 1, the preparation of levamisole mother liquor

[0037] Commercially available veterinary levamisole hydrochloride injection with a levamisole content of 50 mg / ml was used as the levamisole mother solution; it was filtered and sterilized with a 0.22 μm filter (purchased from Sartorius) before use.

[0038] 2. Preparation of aqueous solution

[0039] Get 20ml of 50mg / ml levamisole mother solution and mix with 80ml chicken Newcastle disease inactivated virus liquid, and adjust the pH value to reach neutrality, be the aqueous phase solution that contains levamisole in every milliliter of solution and be 10mg.

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Abstract

The invention discloses a vaccine adjuvant and an application thereof in preparation of a Newcastle disease inactivating vaccine, and belongs to the field of the preparation of a Newcastle disease inactivating vaccine. The invention discloses a method for preparing the Newcastle disease inactivating vaccine by virtue of the vaccine adjuvant. The method comprises the following steps: (1) proliferating Newcastle disease viruses, collecting a virus solution and inactivating the virus solution; (2) adding a levomisole mother liquid to the inactivated virus mother liquid and uniformly mixing so as to obtain an aqueous phase solution; (3) adding a surfactant to the aqueous phase solution and uniformly mixing; and (4) adding white oil and emulsifying so as to obtain the Newcastle disease inactivating vaccine. The Newcastle disease inactivating vaccine prepared by the method disclosed by the invention is good in safety, and the vaccine can stimulate a body to generate relatively strong cellular immunity and humoral immunity and prolong the immune protection period thereof.

Description

technical field [0001] The invention relates to a method for preparing an inactivated Newcastle disease vaccine by using an adjuvant, and also relates to an inactivated Newcastle disease vaccine prepared by the method, belonging to the field of inactivated Newcastle disease vaccine preparation. Background technique [0002] Adjuvant (adjuvant), also known as immune adjuvant, its function is to mix with the antigen and inject it into the animal body, which can non-specifically change or enhance the body's specific immune response to the antigen, and exert its adjuvant effect. Any substance that can enhance the antigen-specific immune response can be called an adjuvant. There are many types of adjuvants, and conventional adjuvants include aluminum salts, oil emulsions, and propolis adjuvants. The most common adjuvants are oil adjuvants, such as Freund's adjuvant, white oil adjuvant, MF-59, SAF, etc. These adjuvants usually have a good immune enhancing effect. [0003] At pre...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/17A61P31/14
Inventor 冯建民颜彤王国辉王石何玉友张影刘斌杨键李长艳廉维杨泽彬冯会柳志光白杨吴艳丽田丽华于志云曲红李海
Owner JILIN ZHENGYE BIOLOGICAL PROD
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