Preparation method of compound photosynthetic bacterial fertilizer

A photosynthetic bacterial fertilizer and seed technology, which is applied in the field of compound photosynthetic bacterial fertilizer preparation, can solve the problems of open technical scheme of the preparation method of peach red sulfur bacterial fertilizer, unfavorable large-scale application, and slow growth rate, so as to improve the quality of agricultural products, Effects that promote transformation and circulation, and promote growth

Inactive Publication Date: 2015-06-03
北京聚益成广科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But to put it into practical use, the first problem to be solved is that the growth rate of the bacteria is slow, and it usually takes two weeks to cultivate for a clear growth trend. It can be used as a bacterial agent after 20-40 days of cultivation, and the value of OD650 Not higher than 1, the live bacteria content is lower than 100 million / ml, and the long growth cycle is not conducive to large-scale application
And up to the present, also do not see the disclosure of the technical scheme of the preparation method of the relevant peach tree sulfur bacterium fertilizer

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] A kind of preparation method of composite photosynthetic bacteria fertilizer adopts the Sulfur pericum strain in the purple sulfur photosynthetic bacteria and the Rhodopseudomonas sphaericus in the purple non-sulfur photosynthetic bacteria to cultivate according to the following steps:

[0059] 1. Activation culture of the semi-solid seeds of Thiobacillus tarotrichum: puncture the species of Thiobacillus tarotrichum in the semi-solid medium of Thiobacillus rubrum, and culture it under light at 30°C for 9 days. It may be an activated Sulfur pericum species;

[0060] 2. Plate activation of Rhodopseudomonas spheroides: Streak the strains of Rhodopseudomonas spheroids on the plate to activate, activate and cultivate at 30°C for 5 days, and pick large colonies as activated seeds;

[0061] . Seed cultivation of Sulfur persicum: inoculate the activated strains into the liquid culture medium of S. persicum seeds, at a temperature of 30°C, at a light intensity of 1500 lux, f...

Embodiment 2

[0070] A kind of preparation method of composite photosynthetic bacteria fertilizer adopts the Sulfur pericum strain in the purple sulfur photosynthetic bacteria and the Rhodopseudomonas sphaericus in the purple non-sulfur photosynthetic bacteria to cultivate according to the following steps:

[0071] 1. Activation and cultivation of the semi-solid seeds of Thiobacillus tarotrichum: puncture the species of Thiobacillus tarotrichum in the semi-solid medium of Thiobacillus tarotrichum, and culture it under light at 30°C for 8 days, and the bacterium thread to be punctured turns red and grows a lawn, that is It may be an activated Sulfur pericum species;

[0072] 2. Plate activation of Rhodopseudomonas spheroides: Streak the strains of Rhodopseudomonas spheroids on the plate to activate, activate and cultivate at 30°C for 5 days, and pick large colonies as activated seeds;

[0073] . Seed cultivation of Sulfur persicum: inoculate the activated strains into the liquid cultur...

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Abstract

The invention provides a preparation method of a compound photosynthetic bacterial fertilizer, and particularly provides a preparation method of the compound photosynthetic bacterial fertilizer obtained after mixed culture of thiocapsa roseopersicina and rhodopseudomonas sphaeroides which are high in growth speed, high in thallus content and short in culture time. A thiocapsa roseopersicina strain of purple sulfur photosynthetic bacteria and a rhodopseudomonas sphaeroides strain of purple non-sulfur photosynthetic bacteria are subjected to culture steps such as seed activation culture, seed culture, illumination culture in an anaerobic fermentation tank and the like, and the compound photosynthetic bacterial fertilizer containing thiocapsa roseopersicina and rhodopseudomonas sphaeroides is obtained. The compound photosynthetic bacterial fertilizer can realize disease prevention, fertilization, medicinal residue degradation and soil remediation, takes effect fast and has a stable effect, and the use amount is small.

Description

technical field [0001] The invention belongs to the technical scope of microbial bacterial fertilizer, and in particular relates to a preparation method of composite photosynthetic bacterial fertilizer. Background technique [0002] Photosynthetic bacteria are listed as Bacteria, Eubacteria, Rhodospirillales, subdivided into Rhodospirillae and Chlorobacteriaceae, and Rhodospirillales are further divided into Rhodospirillaceae (purple non-sulfur photosynthetic bacteria) and Rhodospirillales. Chromobacteriaceae (purple sulfur photosynthetic bacteria). Photosynthetic bacteria belonging to the order Rhodospirillum can perform photosynthesis under anaerobic conditions without releasing oxygen. Cells contain bacteriochlorophyll and various carotenoids, as well as cytochromes, ubiquinone, and iron redox-type non-heme ferritins. According to the eighth edition of "Berger's Handbook of Bacteria", the photosynthetic bacteria of Rhodospiraceae can photosynthesize simple organic subst...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08C12N1/20C12R1/01
CPCY02W30/40
Inventor 蒋常德
Owner 北京聚益成广科技有限公司
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