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A kind of algae natural anti-algae agent and its preparation method and application

An algae inhibitor, a natural technology, applied in the fields of algae, ecology and marine biology, can solve the problems of high cost, secondary pollution by chemical methods, low efficiency of physical methods, etc., and achieve high algae inhibitory activity and good inhibitory effect. , the effect of eliminating secondary pollution

Active Publication Date: 2017-06-27
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a variety of algae control methods have been proposed at home and abroad, but there are drawbacks. Most of the physical methods are inefficient and expensive; chemical methods are prone to secondary pollution.

Method used

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  • A kind of algae natural anti-algae agent and its preparation method and application
  • A kind of algae natural anti-algae agent and its preparation method and application
  • A kind of algae natural anti-algae agent and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of algae inhibitor:

[0035] Specific steps are as follows:

[0036] After activating Protocercia reticulae species, insert it into L1 medium in a sterile room, the size of the culture bottle is 1 liter, the temperature of the incubator is (20±1°C), and the light-dark ratio is L:D=12h: 12h, continue to cultivate to the exponential growth phase; in the sterile room, insert Protocercia reticulum in the exponential growth phase into the L1 medium, the inoculation density is 5000cells / mL, the size of the culture bottle is 1 liter, and the culture temperature is (20 ±1°C), the light-to-dark ratio is L:D=12h:12h, cultured for 10 days, and the active algae liquid was obtained, which was determined to contain 1 unit of algastatic agent activity, that is, the density was 5000cells / mL Protochaeter reticulum in 1 The amount of algastatic agent obtained by culturing in liter medium for 10 days; filter the active algae liquid through a 5 μm filter membrane to remove alg...

Embodiment 2

[0038] Allelopathic inhibitory effect of active algal liquid, cell-free filtrate and powder preparation of Protocercia reticulae on several common red tide algae

[0039] 1) The logarithmic growth phase Chaetoceros pseudospirulina, Karenia mirkei, Heterocurvium akashiwo and Prorocentrum donghaiense were selected as recipient algae species, with a cell density of 5000 cells / mL.

[0040] 2) Insert four kinds of red tide algae into the L1 culture medium respectively containing the active algae liquid of Protocercia reticulum, the cell-free filtrate and the powder preparation, and these culture media all contain 1 active unit of algae-inhibiting ingredients (implementation obtained in Case 1).

[0041] 3) In addition, the same number of several recipient algae species were inserted into the normal L1 medium as a blank control, a total of 4 groups of samples, and one control was set for each group.

[0042] 4) Continuously culture for 10 days and take samples every two days, cultu...

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Abstract

The invention relates to a natural algae inhibitor, which is prepared from Protochaeter reticulum. The preparation method of the present invention is simple, the yield is large, the prepared inhibitor has high purity, high activity, and good inhibitory effect. Experiments have proved that the algae inhibitor has a good effect on common red tide algae: Chaetoceros pseudospirulina, Karenia militaris, and Pseudomonas donghaiense. Dinoflagellates and Heterocurvium akashiwo had strong inhibitory effects. The algastatic agent of the invention has obvious advantages in eliminating secondary pollution, can avoid poisoning to other organisms, especially human beings, and can rapidly degrade in the environment. In addition, the powder formulation is convenient for storage and transportation, and also maintains high algae-inhibiting activity.

Description

technical field [0001] The invention relates to the fields of marine biology, ecology and phycology, in particular to a natural algae inhibitor and its preparation method and application. Background technique [0002] In recent years, my country's marine eutrophication has intensified, and red tides have frequently erupted, seriously damaging the health of the marine ecosystem and causing serious economic losses to the aquaculture industry. How to effectively control red tides and algal blooms has become a new goal that people are constantly exploring. At present, various algae control methods have been proposed at home and abroad, but there are disadvantages. Most of the physical methods are low in efficiency and high in cost; chemical methods are prone to secondary pollution. Therefore, it is an important task of current environmental science to explore effective, economical and environmentally friendly methods to control red tides, and it is an urgent need for current ec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/03A01P13/00C12P1/00C02F1/50C12R1/89
Inventor 屈佩庞敏唐学玺
Owner OCEAN UNIV OF CHINA