A kind of preparation method of insulin detemir injection and preparation of insulin detemir injection
A technology for insulin detemir and injections, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, endocrine system diseases, etc., to improve quality, ensure drug safety, and save time
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[0044]Preparation method 1 (preparation method provided by the present invention): (1) take a 1L beaker, add the rotor, place it on a magnetic stirrer, add glycerin and 600mL water for injection, stir to dissolve the glycerin and prepare a glycerin solution; (2) diazepam Insulin, m-cresol, and phenol are pre-dissolved with 180mL, 300mL, and 120mL of the glycerol solution respectively to obtain insulin detemir-glycerol solution, m-cresol-glycerol solution and phenol-glycerol solution; (3) the insulin detemir- Glycerol solution, m-cresol-glycerin solution and phenol-glycerin solution are mixed, stirred evenly, obtain mixed solution I; (4) under stirring, add the sodium hydroxide solution of 0.1mol / L in described mixed solution I to pH= 9.0 to 9.5, so that insulin detemir dissolves rapidly and the solution becomes clear; (5) Take three 25mL beakers, add zinc acetate, sodium chloride and disodium hydrogen phosphate respectively, and add 5mL water for injection to dissolve and prepa...
Embodiment 1
[0048] Example 1 Insulin detemir injection prepared by different methods
[0049] The insulin detemir injection described in this implementation contains insulin detemir 100IU / mL, glycerol 16mg / mL, m-cresol 2.06mg / mL, phenol 1.8mg / mL, zinc 65.4μg / mL, sodium chloride 1.17mg / mL, Disodium hydrogen phosphate 0.89mg / mL, hydrochloric acid, sodium hydroxide and water for injection, pH=7.4; prepare insulin detemir injection according to the above-mentioned preparation method 1, preparation method 2 and preparation method 3, and record the required preparation cycle time, the results are shown in Table 1.
Embodiment 2
[0050] Example 2 Insulin detemir injection prepared by different methods
[0051] The insulin detemir injection described in this implementation contains insulin detemir 100IU / mL, glycerin 17mg / mL, m-cresol 2.2mg / mL, phenol 1.5mg / mL, zinc 65.4μg / mL, sodium chloride 1.1mg / mL, Disodium hydrogen phosphate 0.89mg / mL, hydrochloric acid, sodium hydroxide and water for injection, pH=7.4; prepare insulin detemir injection according to the above-mentioned preparation method 1, preparation method 2 and preparation method 3, and record the required preparation cycle time, the results are shown in Table 1.
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