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Method for selectively activating main beneficial bacteria in oil deposit and keeping high concentration

A selective and beneficial bacteria technology, applied in the field of microbial biotechnology and resources and environment biology, can solve problems such as shortage, and achieve the effect of reducing interfacial tension, increasing the concentration of metabolites, and having obvious emulsification effect.

Active Publication Date: 2015-06-10
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And still lack this technology and nutrition system and corresponding craft at present

Method used

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  • Method for selectively activating main beneficial bacteria in oil deposit and keeping high concentration
  • Method for selectively activating main beneficial bacteria in oil deposit and keeping high concentration
  • Method for selectively activating main beneficial bacteria in oil deposit and keeping high concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Selective activation of the culture effect of a nutrient system for hydrocarbon-oxidizing bacteria. The nutrient solution formula of this system is: the aqueous solution contains NaNO 3 0.4%, NH 4 Cl 0.1%, sodium polyphosphate 0.12%, auxiliary bacteria solution 3%. The preparation method is: on a clean workbench, after starting the fan for half an hour, take the output liquid to remove oil, then pour 100mL of the output liquid into a 250mL Erlenmeyer flask, and directly add NaNO 3 0.4g, NH 4 Cl 0.1g, sodium polyphosphate 0.12g, auxiliary bacteria solution 3, prepared as culture bacteria solution, add crude oil 2g. The preparation method of other selective activation nutrient solutions is the same.

[0044] Culture results show ( figure 1 ): The number of aerobic hydrocarbon oxidizing bacteria was on the rise due to some oxygen in the shake flask at the initial stage of culture, and gradually decreased until it disappeared with the prolongation of culture time,...

Embodiment 2

[0051] Selective activation of the culture effect of a nutrient system for hydrocarbon-oxidizing bacteria. The nutrient solution formula of this system is: the aqueous solution contains NaNO 3 0.7%, NH 4 Cl 0.3%, sodium polyphosphate 0.2%, auxiliary bacteria solution 5%.

[0052] The selective hydrocarbon oxidizing bacteria nutrient system cultivation experiment was carried out according to the laboratory experiment method overview, and the results showed that the results were similar to those in Example 1. This is because within the optimal range of microbial nutrient concentration, the results of microbial culture for the same output fluid are similar. This is consistent with Wang Dawei's "Biotechnology Bulletin" in the article "Exogenous and endogenous microorganisms in the process of nutrient activation process and their effect on crude oil". Only one optimal nutrient formula concentration is given.

Embodiment 3

[0054] Selectively activate the cultivation effect of the fermented bacteria nutrient system. Based on the experimental results of Example 1 and Example 2, the nutrient solution culture liquid of this system only gives the culture result of one formula. The nutritional system for selective activation of fermented bacteria consists of: NaNO in aqueous solution 3 0.7%%, NH 4 Cl-0.5%, sodium polyphosphate 0.08%, powdered sugar 0.8%, compound nutrient 0.3%.

[0055] Culture results ( image 3 ) showed that in the continuous culture process, during the transition from aerobic to anaerobic consumption, the concentration of anaerobic fermentation bacteria increased slowly in the first 7 days, and as the oxygen was consumed, the bacterial concentration increased rapidly in 12 days and then in 20 days The number of hydrocarbon oxidizing bacteria and methanogenic bacteria increased, but they were not selectively activating the target bacteria, so the number of bacteria was small. T...

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Abstract

The invention discloses a method for selectively activating main beneficial bacteria in an oil deposit and keeping high concentration. In the method, through continuous and alternate injection of different selective nutrient solution slugs and addition of auxiliary emulsifying bacteria and composite nutrients into the different slugs, microbial activations and crude oil acting effects in different stages are intensified. The auxiliary bacteria promote hydrocarbon oxidizing bacteria to emulsify crude oil ahead of time, and the composite nutrients increase the growth rates and the metabolite concentrations of fermenting bacteria and methanogenses, so that oil / water interfacial properties are improved, the swept volume of an injected fluid is increased, and further the crude oil recovering rate is improved; in the method, growth and metabolism data of the main oil recovering florae under the condition of different optimal formulae and the injection amounts of different main nutrient solution slugs are provided, and a proper oil deposit condition and the minimum scale are provided; the method is applied to Dongxin oilfield on site, and application results show that the method has a relatively good effect of controlling the number of microbes and the metabolite concentration, and has significant staged oil increasing and water reducing effects.

Description

technical field [0001] The invention relates to a method for selectively activating main beneficial bacteria in oil reservoir endogenous microorganisms and maintaining its height, increasing the concentration of metabolites, improving the interface properties between crude oil and displacement, and improving the effect of microbial oil displacement, belonging to microbial biotechnology and Resources and environment biotechnology field. Background technique [0002] Oilfields that have entered the high water cut period are facing problems such as decreasing resources, difficult mining, and high mining costs. All countries are working to improve the oil displacement efficiency of the developed blocks and expand the swept volume of injected liquid to further increase the recovery rate. Among the many enhanced oil recovery technologies, microbial oil recovery technology has the advantages of low cost, strong adaptability, low damage to production layers, and no environmental pol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): E21B43/22
CPCE21B43/16
Inventor 马挺李国强高配科
Owner NANKAI UNIV
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