Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extracting luteolin and β-sitosterol from peanut shells

A technology of luteolin and peanut shells, applied in the direction of steroids, organic chemistry, etc., can solve the problems of high cost, complicated process, and difficult removal of impurities, and achieve low production cost, simple refining method, and reduce the risk of environmental pollution Effect

Active Publication Date: 2017-01-11
HUNAN AGRICULTURAL UNIV
View PDF16 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0019] In summary, there are many preparation methods for luteolin and β-sitosterol. The chemical synthesis method is complex and costly. Due to the introduction of catalysts and other components and by-products, the removal of impurities is very difficult, and the product Security becomes an important issue

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Dry the collected peanut shells in the sun, crush them to 20-40 mesh powder, take 100kg of peanut shell powder, add 400L of 75% ethanol aqueous solution, heat to 45°C for reflux extraction for 3h, filter once with a 0.22μm ceramic membrane to obtain the filtrate, and filter the residue with 75 Repeat the extraction once with 200L of % ethanol aqueous solution, and combine 530L of the filtrate; add 6kg of granular activated carbon to the filtrate for decolorization, and filter through a 0.22μm ceramic membrane to obtain the decolorization filtrate; concentrate the decolorization filtrate under reduced pressure at 40-50°C and -88kPa To dryness, 9.45 kg of dried matter was obtained, and then 15 L of 10% aqueous sodium hydroxide solution was added by mass ratio, and the mixture was evenly mixed and allowed to stand for 25 minutes; after standing, 40 L of ethyl acetate was added to the solution for extraction, and the mixture containing ethyl acetate was obtained. The extract...

Embodiment 2

[0049] Dry the collected peanut shells in the sun, crush them to 20-40 mesh powder, take 200kg of peanut shell powder, add 600L of 70% ethanol aqueous solution, heat to 50°C for reflux extraction for 2.5h, filter once with a 0.22μm ceramic membrane to obtain the filtrate, and use the filter residue Repeat extraction once with 300L of 70% ethanol aqueous solution, and combine 820L of filtrate; add 20kg of granular activated carbon to the filtrate for decolorization, and filter through a 0.22μm ceramic membrane to obtain decolorization filtrate; decompress the decolorization filtrate at 40-50°C and below -88kPa Concentrate to dryness to obtain 21.5kg of dry product, then add 30L of sodium hydroxide aqueous solution with a mass ratio of 11%, mix uniformly and let stand for 20-30min; after standing still, add 40L of ethyl acetate to the solution for extraction, and obtain acetic acid Ethyl extract and an aqueous solution that does not contain ethyl acetate; the extract that contain...

Embodiment 3

[0051]Dry the collected peanut shells in the sun, crush them to 20-40 mesh powder, take 300kg of peanut shell powder, add 1000L of 80% ethanol aqueous solution, heat to 40°C for reflux extraction for 2-5h, and filter once with a 0.22μm ceramic membrane to obtain the filtrate and filter residue Repeat the extraction once with 400L of 80% ethanol aqueous solution, and combine 1300L of the filtrate; add 30kg of granular activated carbon to the filtrate for decolorization, and filter through a 0.22μm ceramic membrane to obtain the decolorization filtrate; decolorize the filtrate at 45 ° C, - 88kPa below the reduced pressure concentration To dryness, 31.8kg of dried matter was obtained, and then 50L of 8% sodium hydroxide aqueous solution was added in a mass ratio, mixed uniformly and then left to stand for 20min; The extract and the aqueous solution not containing ethyl acetate; the extract containing ethyl acetate was concentrated to dryness under reduced pressure to obtain 455g o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for extracting luteolin and beta-sitosterol from peanut shells. The method comprises the following steps: taking the peanut shells as materials, cleaning the peanut shells to remove impurities, crushing the cleaned peanut shells, performing extraction on the crushed peanut shells by an ethanol solution, carrying out filtration, decolorization and concentration under reduced pressure to obtain a luteolin and beta-sitosterol mixture, then mixing the luteolin and beta-sitosterol mixture with an alkali liquor, after that, carrying out extraction separation to obtain beta-sitosterol, performing extraction after pH adjustment to obtain a luteolin crude product, and performing alcohol dissolution, crystallization and recrystallization on the crude product to obtain a luteolin product with relatively high purity. The method is simple to operate, demands less on equipment, lowers the manufacturing cost of luteolin and reduces the risk of environmental pollution.

Description

technical field [0001] The invention relates to a method for extracting luteolin and β-sitosterol by using peanut shells, belonging to the technical field of natural product chemistry. Background technique [0002] Peanut is the second largest edible vegetable oil source and protein resource in my country. The waste peanut shell contains a small amount of lipids, mainly luteolin and β-sitosterol. use. Luteolin has the strongest antioxidant activity, and its antioxidant activity can be used to inhibit the spoilage of oily foods. The results of pharmacological and clinical experiments show that luteolin has antibacterial, antiviral, lower blood lipid and cholesterol, and prevent cardiovascular diseases in vivo; in recent years, a large number of domestic and foreign literatures have reported the antitumor activity of luteolin, and the results show that It has a significant inhibitory effect on a variety of tumors, can interfere with tumor cell metabolism, inhibit cell growth,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40C07J9/00
CPCC07D311/30C07D311/40C07J9/00
Inventor 唐忠海覃静萍
Owner HUNAN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com