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Efficient extraction method for blueberry anthocyanin

A blueberry anthocyanin and high-efficiency technology, which is applied in the direction of organic chemistry and the like, can solve the problems of strong seasonal harvesting and difficult storage of blueberry fruit, and achieves the effects of stable production process parameters, improved extraction rate and high application value.

Inactive Publication Date: 2015-06-24
GUIZHOU INST OF BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Chinese patent document application number CN201010246735.4 discloses a method for extracting blueberry anthocyanins, which uses ethanol acidified with organic acid as the extractant, and avoids corrosion of equipment by strong acid on the premise of ensuring a high extraction rate At the same time, blueberry anthocyanins are extracted from blueberry fruit to solve the problem that blueberry fruit is harvested seasonally and difficult to store

Method used

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  • Efficient extraction method for blueberry anthocyanin

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Spray freshly picked blueberries with 0.02% cellulase at room temperature, and after 3.5 hours in the dark, add citric acid-60% ethanol at a ratio of 1:10 to liquid, continue to stir for 5 hours, and pump Filtration, the filtrate was rotated under reduced pressure to recover ethanol, and the crude extract of blueberry anthocyanins was obtained as a reddish-brown extract.

[0033] Dissolve the above-mentioned blueberry anthocyanin crude extract in an appropriate amount of citric acid solution with pH=3.0, extract with ethyl acetate three times, remove the fat layer, add the non-lipid layer to the DA201 macroporous resin column, and use 4 times the volume of ultrapure water, then eluted with 10% and 30% ethanol in turn, collected the 30% ethanol eluate, and recovered the ethanol by rotating under reduced pressure. The obtained product was freeze-dried to obtain purple-brown blueberry flowers Chin powder. Using 2% HCl-Methanol degradation method, the determination of anth...

Embodiment 2

[0035] The blueberries stored at -40°C and thawed overnight at 0°C were sprayed with 0.04% fruit juice enzyme at room temperature, and after 4 hours in the dark, citric acid-50% ethanol with pH=3.5 was added according to the ratio of solid to liquid 1:15 , continue to stir for 4 hours, filter with suction, spin the filtrate under reduced pressure to recover ethanol, and obtain the crude extract of blueberry anthocyanins, which is a purple-red extract.

[0036] Dissolve the above blueberry anthocyanin crude extract in an appropriate amount of hydrochloric acid solution with pH=1.0, extract with ethyl acetate for 3 times, remove the lipid layer, add the non-lipid layer to the AB-8 macroporous resin column, first use 3 times the volume of ultrapure water for elution, and then sequentially eluted with 10%, 25%, and 45% ethanol, collected 45% ethanol eluate, and recovered ethanol by rotating under reduced pressure. The obtained product was freeze-dried to obtain purple Brown bluebe...

Embodiment 3

[0038] Spray freshly picked blueberries with 0.02% cellulase-pectinase at room temperature. After 3.5 hours in the dark, add citric acid-70% ethanol with a pH=3.0 ratio of solid to liquid at a ratio of 1:8 and continue stirring. After 6 hours, suction filtration, the filtrate was rotated under reduced pressure to recover ethanol, and the crude extract of blueberry anthocyanins was obtained as a reddish-brown extract.

[0039] Dissolve the above blueberry anthocyanin crude extract in an appropriate amount of citric acid solution with pH=3.5, extract with ethyl acetate for 3 times, remove the lipid layer, add the non-lipid layer to D101 macroporous resin column, first use 4 Double the volume of ultrapure water for elution, then eluted with 10%, 25%, and 50% ethanol in sequence, collected 50% ethanol eluate, recovered ethanol by rotating under reduced pressure, and freeze-dried the obtained product to obtain purple-brown blueberry anthocyanin powder. Using 2% HCl-Methanol degrad...

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Abstract

The invention discloses an efficient extraction method for blueberry anthocyanin. According to the method, blueberry fruits or blueberry leaves are taken as raw materials for extracting the blueberry anthocyanin and the method comprises extraction and purification of a blueberry anthocyanin crude extract and specifically comprises the following steps of carrying out enzymolysis and alcohol extraction in turn to obtain the blueberry anthocyanin crude extract, then purifying the blueberry anthocyanin crude extract, degreasing by extraction and removing impurities with macroporous resin to obtain the refined blueberry anthocyanin. According to the method disclosed by the invention, solvent extraction and enzyme extraction are simultaneously combined, enzymolysis is firstly used for improving the juice yield of the blueberry anthocyanin and a solvent is further used for extracting the blueberry anthocyanin. By adopting the extraction method, the extraction time can be greatly shortened, the active ingredients can be extracted to the greatest extent, and the extraction rate can be significantly improved; furthermore, the operation is simple and easy to operate, the parameters of the production process are stable, the product quality is stable, and the extraction method is suitable for industrial production; and in addition, the highest content of an extracted and purified blueberry anthocyanin fine product can achieve 49.6%, and the application value is very high.

Description

technical field [0001] The invention relates to the technical field of substance extraction, in particular to a method for efficiently extracting blueberry anthocyanins, so as to realize the efficient extraction and purification method of blueberry anthocyanins. Background technique [0002] Blueberry anthocyanin is one of the main active components of blueberry, which has strong antioxidant properties, and has various functions such as promoting resynthesis of retinoid, anti-inflammation, improving immunity, anti-cardiovascular disease, anti-aging, anti-cancer, etc. Physiologically active function has been highly concerned by experts at home and abroad, and has become one of the hot spots in blueberry research. [0003] The extraction of blueberry anthocyanins is the premise of blueberry research and development, and it is also a key link in the production and use of anthocyanins. In the research on the extraction of blueberry anthocyanins, the existing technologies mainly...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 徐青贾强韩瑜周元敬屈小媛聂飞
Owner GUIZHOU INST OF BIOLOGY
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