Reagent and method for detecting MGMT gene promoter methylation
A technology for detection of reagents and promoters, applied in the fields of life science and biology, can solve problems such as variation of CG content and TM value, influence on acquisition, etc., and achieve high detection rate, high accuracy, and high throughput
Inactive Publication Date: 2015-06-24
FUZHOU ADICON CLINICAL LAB INC
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The invention discloses a reagent and a method for detecting MGMT gene promoter methylation. The reagent comprises (1) specific amplimers as shown in SEQ NO 1, SEQ NO2 of which the 5th ends are marked with biotin, as well as specific sequencing primer SEQ NO3; and (2) a PCR amplified reaction reagent, a sulfite treatment reagent and a pyrophosphoric acid sequencing related reagent. The reagent and the method disclosed by the invention have the advantages of short detection cycle, high specificity, high accuracy, low pollution risk and the like, and can be used for judging the MGMT gene promoter methylation state of a to-be-detected sample, and the detection result is beneficial to prediction of early-phase related tumor diseases, and can be used for guiding clinical doctors to use alkylating agent medicines according to individual differences of patients so as to realize individual treatment and for guiding doctors to carry out prognosis treatment of related diseases.
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