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Method for preparing biodiesel by directly performing ester exchange by oleaginous microorganisms

A technology of oil-producing microorganisms and biodiesel, which is applied in the fields of biofuel, fat production, fat oil/fat refining, etc., can solve the problems of high toxicity, high energy consumption, environmental impact, etc., and achieve easy operation, simple process flow, and energy saving consumption effect

Inactive Publication Date: 2015-07-01
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But above-mentioned method all has deficiencies, and the energy consumption of using ultrasonic wave, microwave is very big, is not easy to industrialization; Although the wall breaking effect of chloroform is very good, toxicity is very big, has certain influence to environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Weigh 0.3g of dry algae powder of Chlorella in a 50mL round bottom flask, add 6mL of a mixed solution of n-hexane and 75% ethanol (n-hexane: 75% ethanol=1:2 (v / v), 0.8mL Concentrated sulfuric acid, place the round bottom flask in a constant temperature oil bath at 90°C to condense and reflux, and stir for 120 minutes. After the reaction is completed, take out the reacted solution, leave it to cool at room temperature for 1 hour, and filter to remove the algae powder. The filtrate was transferred to a separatory funnel, extracted with 4mL of n-hexane, added 4mL of saturated NaCl solution, and after standing for stratification, the upper n-hexane phase was collected, extracted repeatedly for 3 times, the organic phases were combined, and distilled under reduced pressure to obtain biodiesel crude oil. Product, the conversion yield of biodiesel is 90.61%.

Embodiment 2

[0023] Weigh 0.3g of dry algae powder of chlorella in a 50mL round bottom flask, add 4.5mL of a mixed solution of n-hexane and 75% ethanol (n-hexane: 75% ethanol=1:2 (v / v), 0.6mL Condensed sulfuric acid, put the round bottom flask in a constant temperature oil bath at 90°C to condense and reflux, and stir for 120 minutes. After the reaction is completed, take out the reacted solution, let it stand and cool at room temperature for 1 hour, and filter to remove the algae powder , the filtrate was transferred to a separatory funnel, extracted with 3mL of n-hexane, added 3mL of saturated NaCl solution, after standing for stratification, the upper n-hexane phase was collected, extracted repeatedly 4 times, the organic phase was combined, and distilled under reduced pressure to obtain biodiesel The conversion yield of crude product, biodiesel is 84.46%.

Embodiment 3

[0025] Weigh 0.3g of dry algae powder of Chlorella in a 50mL round bottom flask, add 6mL of a mixed solution of n-hexane and 75% ethanol (n-hexane: 75% ethanol=1:2 (v / v), 0.4mL Concentrated sulfuric acid, put the round-bottomed flask in a constant temperature oil bath at 100°C to condense and reflux, and stir for 120 minutes. After the reaction is completed, take out the reacted solution, leave it to cool at room temperature for 1 hour, and filter to remove the algae powder. The filtrate was transferred to a separatory funnel, extracted with 4mL of n-hexane, added 4mL of saturated NaCl solution, and after standing for stratification, the upper n-hexane phase was collected, extracted repeatedly for 3 times, the organic phases were combined, and distilled under reduced pressure to obtain biodiesel crude oil. The product, the conversion yield of biodiesel is 89.13%.

[0026] In the above examples, the oleaginous microorganisms used were replaced by Staphylococcus, Scenedesmus, Du...

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Abstract

The invention discloses a method for preparing biodiesel by directly performing ester exchange by oleaginous microorganisms. The method is used for preparing biodiesel by directly performing ester exchange by taking oleaginous microorganisms as raw materials. The method comprises the following steps: mixing a mixed solvent comprising dried biomass with normal hexane and alcohol with certain concentration with a catalyst concentrated sulfuric acid, and performing reaction under reflux condensation and stirring for a certain time in a constant-temperature oil bath at a certain temperature; after the reaction is ended, filtering to remove filter residues, extracting filtrate, and distilling solvent under reduced pressure to obtain a biodiesel crude product. According to the method disclosed by the invention, a low-toxicity organic solvent is utilized to directly prepare the biodiesel by taking the biomass as a raw material through extraction-ester exchange one step. Assistance of microwaves, ultrasonic sounds and the like is not required in the reaction process, so that the energy consumption is reduced; the solvents are low-toxicity organic solvents, clean and environmentally friendly. The process is simple and easy to operate.

Description

technical field [0001] The invention belongs to the field of biological energy, and specifically relates to a method for preparing biodiesel in one step of extraction and transesterification in an organic solvent system by using oil-producing microorganisms as raw materials and using concentrated sulfuric acid as a catalyst. Background technique [0002] With the increasing depletion of fossil fuels and the pollution of the environment from their combustion, it is urgent to seek sustainable and renewable energy. [0003] Biodiesel (biodiesel) refers to a liquid fuel that can replace petrochemical diesel oil made from animal and vegetable oils, microbial oils, and catering waste oil through transesterification process. It has excellent compatibility with diesel oil and can be mixed with national standard diesel oil. Or used alone, it is a typical clean, renewable "green energy" and an ideal substitute for non-renewable resources such as petroleum. [0004] With the developme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11C3/10C11B3/12C11B3/00C10L1/02
CPCC10L1/02C11B1/10C11B3/001C11C3/10Y02E50/10
Inventor 张栩张艳谭天伟
Owner BEIJING UNIV OF CHEM TECH