Detection method for simultaneous determination of nine water soluble vitamins

A water-soluble vitamin and detection method technology, applied in the field of vitamin detection, can solve problems affecting the accuracy and reproducibility of determination results, increase retention time, vitamin retention time drift, etc., to achieve increased retention time, fast analysis speed, and sensitivity high effect

Inactive Publication Date: 2015-07-01
COFCO NUTRITION & HEALTH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by this invention is that when utilizing high performance liquid chromatography to measure water-soluble vitamins in the prior art, it is necessary to add an ion pair reagent of sodium hexanesulfonate-phosphoric acid in the mobile phase to increase the amount of water-soluble vitamins

Method used

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  • Detection method for simultaneous determination of nine water soluble vitamins
  • Detection method for simultaneous determination of nine water soluble vitamins
  • Detection method for simultaneous determination of nine water soluble vitamins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] In this example, the method of the present invention is used to determine the type and content of water-soluble vitamins in a vitamin functional drink sample, and 1 mL of the functional drink sample is accurately drawn and filtered through a 0.45 μm microporous membrane to inject the sample;

[0070] Wherein, the chromatographic condition of described high performance liquid chromatography is:

[0071] Chromatographic column: Agilent Zorbax Eclipse Plus-C184.6mm×250mm, 5μm;

[0072] Mobile phase A: 10mmol / L phosphate buffer, adjusted to pH=3 with phosphoric acid, mobile phase B: acetonitrile;

[0073] Elution mode: gradient elution, the program of the gradient elution is specifically:

[0074] time / min

mobile phase A

mobile phase B

0

100

0

4

100

0

25

75

25

30

75

25

30.1

100

0

40

100

0

[0075] Column temperature: 30°C;

[0076] Injector temperature: 4°C; ...

Embodiment 2

[0081] The present embodiment uses the method described in the present invention to measure the type and content of water-soluble vitamins in a certain compound vitamin tablet. The specific steps are as follows:

[0082] Take the multivitamin tablet and grind it evenly, accurately weigh 2g of the powder (accurate to 0.0001g), use ultrapure water to make up the volume in a 25mL volumetric flask, ultrasonically extract for 20min, and the ultrasonically extracted solution is filtered with a 0.45μm microporous membrane and injected; Among them, when determining vitamin C, the ultrasonic extraction solution needs to be diluted 20 times with a pH of 3.0 phosphate buffer, and then filtered through a 0.45 μm microporous membrane before sample injection. This is because vitamin C in commercially available multivitamin tablets Therefore, the above-mentioned dilution treatment can prevent the ultraviolet absorption signal value from exceeding the threshold due to the high content of vitam...

Embodiment 3

[0095] In this embodiment, the method of the present invention is used to determine the type and content of water-soluble vitamins in a certain vitamin nutritionally fortified rice, and the specific steps are as follows:

[0096]Take the vitamin-enhanced rice, crush and grind it evenly, accurately weigh 0.2g of the powder (accurate to 0.0001g), add 5mL of pure water, ultrasonically extract for 10min, then place it in a refrigerated centrifuge at 12000rpm / s for 10min at 4°C, transfer to Supernatant liquid; add 2 mL of pure water to dissolve the remaining solid precipitate, ultrasonically extract for 10 min, and then place it in a refrigerated centrifuge at 4°C for 10 min at a speed of 12,000 rpm / s; combine the supernatant liquid after two centrifugations, and add 2 mL of it to a concentration of 0.15 g / mL The ZnSO4 solution was placed in a refrigerated centrifuge at 4°C and centrifuged at a speed of 12000rpm / s for 5min; all the supernatant was transferred to a 10mL volumetric fl...

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Abstract

The invention relates to a detection method for simultaneous determination of nine water soluble vitamins, during determination of water soluble vitamins by use of high performance liquid chromatograph (HPLC), a phosphate buffer-acetonitrile system with the concentration of 10-50mmol / L and pH of 2.0-3.0 is used as a mobile phase, dissociation of water soluble vitamin components can be effectively inhibited in order to increase the retention time of the water soluble vitamin components in a C18 chromatographic column stationary phase, and because of the concentration of the phosphate buffer system is 10-50mmol / L, the mobile phase has great buffer capacity, and when an acidic or alkaline sample is introduced into the chromatographic system, the pH value of the mobile phase can be maintained in no change so as to ensure the separation effect of the water soluble vitamins. The method can realize simultaneous determination of the nine water soluble vitamins, has the characteristics of fast speed, high sensitivity, accurate nature determination and quantification, and is suitable for multicomponent determination of the water soluble vitamins in vitamin nutrition fortified foods.

Description

technical field [0001] The invention relates to a detection method for simultaneously measuring nine kinds of water-soluble vitamins, belonging to the technical field of vitamin detection. Background technique [0002] Vitamins are divided into two types: water-soluble and fat-soluble. Water-soluble vitamins refer to vitamins that can be dissolved in water, usually as a component of coenzymes or prosthetic groups, including B vitamins and vitamins that play an important role in the catalysis of enzymes. C et al. Due to the large structural differences and unstable chemical properties of different water-soluble vitamins, it is difficult to achieve simultaneous separation and determination. Traditional analysis methods for water-soluble vitamins include ultraviolet spectrophotometry, fluorescence photometry, and microbial methods, etc. Most of them are based on the determination of a single vitamin. The influence of interference factors is large, the sensitivity is low, and ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 谢云峰任丹丹杨永坛刘佳佳
Owner COFCO NUTRITION & HEALTH RES INST
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