Three dimensional biology printing device and method

Abstract

The invention discloses a three dimensional biology printing device and a three dimensional biology printing method. The three dimensional biology printing device comprises a printing platform, a spray head, a spray head electric controller, two CCD (charge coupled device) optical systems with light sources, a plasma sterilization mechanism, a motion control system, a pneumatic control mechanism, a humidity regulator, a temperature control unit, a liquid storage tank, an open container which can contain a nutrient solution, an installation frame and a housing, wherein the installation frame is arranged in the housing and higher than the middle of the housing, the spray head is arranged on the installation frame, the optical systems are respectively arranged above the spray head and on a portion of the installation frame, on the left side of the spray head, the open container is arranged in the housing, the printing platform is arranged in the open container, a nozzle of the spray head faces downwards, and corresponds to the printing platform, the pneumatic control mechanism is installed on the exterior of the housing, and all the other units except the pneumatic control mechanism are located in the housing. The invention further provides the method using the three dimensional biology printing device to print a model. The three dimensional biology printing device does not need to be placed in a clean work room for use, is low in cost, can control a device environment, and is suitable for various biological materials and cells.

Description

technical field [0001] The invention relates to the technical field of bioprinting in tissue engineering, in particular to the field of a three-dimensional bioprinting device and a bioprinting method based on the three-dimensional bioprinting device. Background technique [0002] 3D printing technology is a new molding technology based on the idea of ​​discrete and stacked molding. It can quickly and accurately manufacture the solid model of a part or object based on the 3D model data of the part or object. With the introduction of the concept of biomanufacturing, 3D bioprinting technology is also playing an increasingly important role in the medical field. 3D bioprinting technology is based on the computer 3D design model, through the method of software layered discrete and numerical control molding, through the controlled assembly of material droplets and cell material units, to design and manufacture the required biologically active artificial tissues and organs , medica...

AI Technical Summary

Problems solved by technology

The disadvantage of this device is that: the introduction of cross-linking agent, the cross-linking agent exists on the surface of the cured material unit, resulting in unreliable connection between the manufactured three-dimensional structure layer and the layer, and it is difficult to form a multi-layer cell three-dimensional structure with a high height. And the mechanical strength of the formed structure is not high

The disadvantage of this device is that: the cold air unit and the refrigeration unit or the cold source unit and the refrigeration unit need to continue to work and refrigerate during the printing process, which consumes a lot of energy, and the cold air unit and the refrigeration unit are connected to the printing nozzle in the device, which has great impact on the mechanical properties of the device. The structural strength has high requirements, which is not conducive to the structural stability of long-term work

[0005] At present, the bioprinting device developed according to the three-dimensional bioprinting technology still has the following limitations: 1. The diameter of the micro nozzle used in the extrusion molding technology is 30-60um, which is similar to the diameter of the cell. prone to clogging during printing

2. The bioprinting device does not consider the working environment during the printing process. There are harmful components, various viruses and allergens in the air, which will directly affect the biological activity of cells

3. The bioprinting device is limited by the structure and materials, and the scope of use is limited

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