A composition for improving sleep and its preparation method
A technology for improving sleep and composition, which is applied in the field of sleep improving composition and its preparation, can solve the problems of lack of energy, increase the burden on the liver and kidney, and the effect is not confirmed, so as to improve sleep, increase the incidence of falling asleep, Toxic effects
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Embodiment 1
[0092] Example 1 Preparation of freeze-dried apple stem cell powder
[0093] 1. The formula of the culture medium:
[0094] 1.1 Induction medium: MS solid medium+NAA0.5mg / L+BA2mg / L+CH 1mg / L, pH 6.0;
[0095] 1.2 Proliferation medium: MS liquid medium+2,4-D 2.0mg / L+BA1.0mg / L+CH1.0mg / L+AC1.0mg / L, pH value is 6.0;
[0096] 2. Sterilization of apple branches
[0097] Choose apple trees that are not polluted by chemical fertilizers and pesticides and are planted green, and select 10 new shoots of 10cm in length according to certain criteria; wash the surface attachments of the new shoots with 2L of sterile water; soak the new shoots in 120mg / L L-ascorbic acid 1 minute; sterilize the new shoots in 75% ethanol solution for 1 minute and rinse them with 2L sterile water; then sterilize them in 30% hydrogen peroxide solution for 20 minutes, and rinse with sterile water for 10 minutes.
[0098] 3. Induction of cambium
[0099] Use an inoculating knife to peel off the cambium, phloem, cortex and epi...
Embodiment 2
[0108] Example 2 Preparation of sleep-improving composition
[0109] First, 100g of freeze-dried apple stem cell powder, 100g of jujube seed extract, and 40g of γ-aminobutyric acid were pulverized (the freeze-dried apple stem cell powder was ground with liquid nitrogen, and the extract of jujube seed and γ-aminobutyric acid were subjected to ordinary Grind), pass a 100-mesh sieve, and then mix the crushed and sieved materials in a mixer.
Embodiment 3
[0110] Example 3 Preparation of sleep-improving composition
[0111] Firstly, 100g of freeze-dried apple stem cell powder, 100g of jujube seed extract, and 75g of γ-aminobutyric acid were pulverized. Grind), go through a 100-mesh sieve, and then mix the crushed and sieved materials in a mixer.
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