Kit and method for detecting methylation level of liver cancer risk gene TSPYL5

A methylation and kit technology is applied in the field of kits for detecting the methylation level of liver cancer risk gene TSPYL5, which can solve the problems of time-consuming, capital and energy-consuming, expensive and cumbersome process.
CN104762301AActive Publication Date: 2015-07-08WUHAN UNIV
0 Cites 16 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN UNIV
Publication Date
2015-07-08

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a kit and a method for detecting the methylation level of liver cancer risk gene TSPYL5, and belongs to the field of biotechnologies. The kit for detecting the methylation level of the TSPYL5 comprises a primer pair A used for an MSRE-qPCR method or a primer pair B used for a BSP method, wherein the primer sequence is shown in SEQ ID NO. 1-22. According to the kit and the method disclosed by the invention, the relevance between the methylation of the TSPYL5 gene and liver cancer occurrence is found, and the methylation loci of the TSPYL5 are authenticated; the methylation method for the TSPYL5 by virtue of fluorescent quantitative PCR is established by combining specific methylation sensitive restriction endonuclease with the specific primers; the methylation level of the TSPYL5 is detected through the kit and the method based on MSRE-qPCR, the sensitivity is up to 83.9%, and the specificity is up to 93.25%.
Need to check novelty before this filing date? Find Prior Art

Description

[0001] technical field

[0002] The invention relates to the field of biotechnology, in particular to a method for detecting liver cancer risk genes TSPYL5 Kits and methods for methylation levels. Background technique

[0003] DNA methylation is one of the main contents of epigenetics research. It studies the reversible genetic changes that occur without changing the base sequence of the gene. It is a covalent modification method after DNA molecule replication. In eukaryotes, DNA methylation refers to the transfer of methyl groups to specific bases in DNA molecules using S-adenosylmethionine as a methyl donor under the action of DNA methyltransferases (DNMTs) process, which mainly occurs on cytosines in CpG dinucleotides. In the genome, the regions where the distribution of CpG dinucleotides is relatively concentrated are called CpG islands (CpG islands, CGIs), ranging in size from 100 to 1000 bp, mainly located in the promoter region and the first exon region of genes; th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More