Pseudolarix plant regeneration method using pseudolarix leaves as explant

A technology of pine and explants, which is applied in plant regeneration, botanical equipment and methods, horticultural methods, etc., to achieve the effects of high feasibility, solving the shortage of plant resources and seedling resources, and strong practicability

Inactive Publication Date: 2015-07-15
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on the pine plant mainly focuses on the extraction of the effective components of the pine and the screening of the endophytic fungi, and in the research report of plant biotechnology, there is "a kind of leaf primordium as the The research reports on the culture method of pine callus from explants” (patent number: ZL 201210104866.8) and “a method for suspension culture of pine cells” (patent number: ZL 201210472291.5); however, the above two

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Selection and disinfection of explants: Select healthy growth, no pests and diseases and young leaves that have just been stretched for 1 day on sunny days, put them on the ultra-clean workbench, and use 75% alcohol Disinfect for 10 seconds, rinse with sterile water for 3 times, then disinfect with 0.1% mercuric chloride (5 mL of Tween 20 was added dropwise to 100 mL of 0.1% mercuric chloride solution) for 3 minutes, and finally rinse with sterile water for 2 times Finally, dry the moisture on the surface with sterilized filter paper;

[0022] (2) Processing of explants: put the sterilized leaves on the filter paper, cut the leaves of Pine chinensis with a knife, and the wound is subject to not cutting off the leaves;

[0023] (3) Induction culture of callus: Insert the treated pine leaves into callus induction medium GD+NAA0.5mg / L+GA 3 The callus induction culture was carried out in 0.5mg / L medium, and the leaves began to have callus at the wound on the 7th day wh...

Embodiment 2

[0029] (1) selection and disinfection of explants: select healthy growth, no damage by diseases and insect pests, and young leaves that have just stretched for 2 days in sunny days, put it on the ultra-clean workbench, and use a concentration of 75% Disinfect with alcohol for 15 seconds, rinse with sterile water for 3 times, then disinfect with 0.1% mercuric chloride (7 mL of Tween 20 was added dropwise to 100 mL of 0.1% mercuric chloride solution) for 4 minutes, and finally rinse with sterile water for 4 minutes. blot the moisture on the surface with sterilized filter paper;

[0030] (2) Processing of explants: put the sterilized leaves on the filter paper, cut the leaves of Pine chinensis with a knife, and the wound is subject to not cutting off the leaves;

[0031] (3) Induction culture of callus: horizontally inoculate the treated pine leaves in callus induction medium GD+NAA1.0mg / L+GA 3 1.0mg / L surface for callus induction culture, the leaves began to produce callus at t...

Embodiment 3

[0037](1) Selection and disinfection of explants: select healthy pine leaves without diseases and insect pests, put them on the ultra-clean workbench, disinfect them with 75% alcohol for 30 seconds, and rinse them with sterile water for 3 times Afterwards, sterilize with 0.1% mercuric acid chloride (10 ml Tween 20 was added dropwise to every 100 ml of 0.1% mercuric acid solution) for 6 minutes, and finally rinse with sterile water for 4 times and blot the surface dry with sterilized filter paper. moisture;

[0038] (2) Processing of explants: put the sterilized leaves on the filter paper, cut the leaves of Pine chinensis with a knife, and the wound is subject to not cutting off the leaves;

[0039] (3) Induction culture of callus: Insert the treated pine leaves into callus induction medium GD+NAA1.5mg / L+GA 3 The callus induction culture was carried out in 1.5mg / L medium, and the leaves began to have callus at the wound on the 7th day when they were inserted into the medium, a...

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PUM

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Abstract

The invention discloses a pseudolarix plant regeneration method using pseudolarix leaves as explants. The method comprises the following steps: producing callus through the induction of pseudolarix, performing the steps of bud differentiation culture, rooting culture, seedling hardening and transplanting on the callus in sequence, so as to finally obtain pseudolarix tissue culture seedlings with the high growth speed and the excellent property and without any pest and disease damage. A new path is provided for rapidly propagating the pseudolarix plant in large scale, and the problem that current pseudolarix medicinal material resources are shortage is effectively solved.

Description

technical field [0001] The invention relates to a method for regeneration of pine plants, in particular to a method for regeneration of pine plants using pine leaves as explants. Background technique [0002] The golden pine is an ancient and relict species of Pinaceae endemic to my country. According to the new threatened species classification system standards of the World Conservation Union (IUCN) and the standards of the Chinese Plant Red Book, the golden pine is designated as a endangered species and listed as a national second-class protected plant. [0003] The golden pine is not only an important garden plant, but also an important medicinal plant with broad application prospects. Studies have found that the most important medicinal ingredient of pine pine is the diterpenoid compound hibiscus acid extracted from its bark and root bark. It is the anti-tumor effect that makes the medicinal value of jinsong more prominent. Modern pharmacological research has found th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王跃华唐旭何诗红昝加志马腾香李红芳彭雪婷陈燕何新友朱晓换刘林钟若男胥华李婷婷徐思琴梅英
Owner CHENGDU UNIV
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