Cynomorium songaricum ferment and preparation technology thereof
A technology of yang enzyme and Cynomorium, which is applied in the field of fermentation of herbal medicine Cynomorium, can solve the problems of lack of research
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Embodiment 1
[0054] (1) Take fresh Cynomorium cynomorium, grind it into a slurry, and then sterilize it at low temperature.
[0055] (2) Preparation of wort culture medium: barley malt is screened, washed, and naturally dried to obtain dry malt through acid saccharification, gelatinization, filtration and other processes; 3% glucose is added and sterilized.
[0056] (3) Preparation of fermentation substrate: in the above-mentioned sterilized wort culture medium, add the Cynomorium cynomorium slurry obtained in step (1), and the addition amount of the Cynomorium cynomorium slurry is 3% (v / v) of the culture medium.
[0057] (4) Bacterial plant implantation and fermentation: select lactic acid bacteria as the fermentation strain, expand and cultivate through solid slope culture, and then implant into the fermentation substrate for fermentation. The medium composition of the solid slant culture includes 3% of glucose, 2% of peptone, 1% of soybean powder, 3% of agar, 0.1% of magnesium sulfate, ...
Embodiment 2
[0060] (1) Take fresh Cynomorium cynomorium, grind it into a slurry, and sterilize it with radiation.
[0061] (2) Preparation of apple pulp medium: after peeling and removing cores from apples, it is prepared by washing, beating, filtering, and sterilizing.
[0062] (3) Preparation of fermentation substrate: in the above-mentioned sterilized apple pulp culture medium, add the Cynomorium syringae slurry obtained in step (1), and the addition amount of the Cynomorium syringae slurry is 2% (v / v) of the medium.
[0063] (4) Bacterial seed implantation and fermentation: Lactobacillus bulgaricus was selected as the fermentation strain, and after solid slant culture and seed tank culture for expanded culture, it was implanted into the fermentation substrate for fermentation. The medium composition of the solid slant culture includes 3% of glucose, 1% of peptone, 1% of bean flour, 2% of agar, 0.06% of magnesium sulfate, 0.2% of potassium dihydrogen phosphate, and 0.1% of sodium chlor...
Embodiment 3
[0066] (1) Take fresh Cynomorium cynomorium, grind it into a slurry, and chemically sterilize it.
[0067] (2) Preparation of corn aqueous solution culture medium: remove impurities, wash and grind corn, add water to boil, filter, add 5% glucose, and sterilize.
[0068] (3) Preparation of fermentation substrate: In the above-mentioned sterilized grain aqueous solution culture medium, add the Cynomorium cynomorium slurry obtained in step (1), and the addition amount of the Cynomorium cynomorium slurry is 5% (v / v) of the culture medium.
[0069] (4) Bacterial seed implantation and fermentation: Lactobacillus acidophilus is selected as the fermentation strain, and after being expanded by shake flask culture and seed tank culture, it is implanted into the fermentation substrate for fermentation. The medium composition of the shake flask culture includes 2% of glucose, 3% of peptone, 2% of bean flour, 1% of bran, 0.5% of magnesium sulfate, 0.2% of potassium dihydrogen phosphate and...
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