Cell inoculation method

Abstract

The invention provides a cell inoculation method and belongs to the field of microalgae cultivation. In the process of adhesive culture of microalgae, the method can be used for solving the problem that a breeding carrier is just limited to a porous and multi-fluff material and expands the material selection range of the breeding carrier, and moreover, certain carrier materials which are strong in durability and low in cost can be further suitable for microalgae cultivation. The method comprises the following steps: preparing a cell solution with a to-be-inoculated concentration of 0.5-300g / L; pre-placing a carrier; adhering the cell solution with the to-be-inoculated concentration of 0.5-300g / L to the carrier, wherein prepation of the cell solution with the to-be-inoculated concentration of 0.5-300g / L comprises the step of culturing cells to a stage of self-secreting viscous matters and / or before adhering the cell solution with the to-be-inoculated concentration of 0.5-300g / L to the carrier, the method comprises the step of pre-treatment of increasing adhesiveness of the carrier on the carrier. The method provided by the invention can be used for cell inoculation.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to a cell inoculation method. Background technique [0002] The large-scale and high-yield cultivation of microalgae is the bottleneck restricting the industrialization of microalgae, and the development of low-cost and high-efficiency cultivation methods is the key to large-scale production. For this reason, people have actively explored a new culture method with great potential for expansion—"attachment culture", which is a way to attach microalgae to a medium for immobilized culture. [0003] For attachment culture, algae cells grow on the surface of the medium. The key issue is whether the cells can be firmly attached to the culture carrier. If the adhesion strength is not good, the cells will fall off and affect the culture yield. The problem of adhesion restricts the selection and application range of algae species and carrier materials in the breeding process. In order ...

AI Technical Summary

Problems solved by technology

[0004] The invention provides a cell inoculation method, which solves the problem that the culture carrier can only be limited to porous and fluffy materials during the attachment culture process of microalgae cells, expands the material selection range of the culture carrier, and can also make some other Durable, low-cost carrier material also suitable for microalgae farming