Cell inoculation method

A cell and cell liquid technology, applied in the field of microalgae cultivation, can solve the problem that the cultivation carrier can only be limited to porous and fluffy materials, and achieve the effect of expanding the selection range and enhancing the adhesion strength

Inactive Publication Date: 2015-07-15
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides a cell inoculation method, which solves the problem that the culture carrier can only be limited to porous and fluffy materials during the attachment culture p

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0055] Example 1

[0056] Use the water culture method to cultivate the chlorella cells to a concentration of 0.6g / L; preset a glass plate as a culture carrier; evenly smear the water binder on the glass plate carrier. The algae cells were inoculated onto the glass plate by circulating spray. After culturing for 48 hours, some algae cells adhered to the glass plate and fell off to a cell concentration of 0.4g / L in the reservoir at the bottom of the reactor.

Example Embodiment

[0059] Example 2

[0060] Use the water culture method to cultivate the chlorella cells to a concentration of 0.8g / L; preset a wooden board as a culture carrier; scratch the wooden board to increase the surface roughness of the carrier. The chlorella cells are evenly attached to the wood board by spraying to complete the inoculation. After culturing for 48 hours, a certain amount of algae cells were attached to the wooden board and fell off to a cell concentration of 0.68 g / L in the reservoir at the bottom of the reactor.

Example Embodiment

[0063] Example 3

[0064] Use the water culture method to cultivate the chlorella cells to a concentration of 1g / L; preset a plastic plate as a culture carrier; apply a surfactant to the surface of the plastic plate to increase the hydrophilicity of the carrier. The chlorella was inoculated onto the plastic plate by circulating spray. After culturing for 48 hours, a certain amount of algae cells were attached to the glass plate and fell off to the cell concentration of 0.58 g / L in the reservoir at the bottom of the reactor.

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PUM

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Abstract

The invention provides a cell inoculation method and belongs to the field of microalgae cultivation. In the process of adhesive culture of microalgae, the method can be used for solving the problem that a breeding carrier is just limited to a porous and multi-fluff material and expands the material selection range of the breeding carrier, and moreover, certain carrier materials which are strong in durability and low in cost can be further suitable for microalgae cultivation. The method comprises the following steps: preparing a cell solution with a to-be-inoculated concentration of 0.5-300g/L; pre-placing a carrier; adhering the cell solution with the to-be-inoculated concentration of 0.5-300g/L to the carrier, wherein prepation of the cell solution with the to-be-inoculated concentration of 0.5-300g/L comprises the step of culturing cells to a stage of self-secreting viscous matters and/or before adhering the cell solution with the to-be-inoculated concentration of 0.5-300g/L to the carrier, the method comprises the step of pre-treatment of increasing adhesiveness of the carrier on the carrier. The method provided by the invention can be used for cell inoculation.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to a cell inoculation method. Background technique [0002] The large-scale and high-yield cultivation of microalgae is the bottleneck restricting the industrialization of microalgae, and the development of low-cost and high-efficiency cultivation methods is the key to large-scale production. For this reason, people have actively explored a new culture method with great potential for expansion—"attachment culture", which is a way to attach microalgae to a medium for immobilized culture. [0003] For attachment culture, algae cells grow on the surface of the medium. The key issue is whether the cells can be firmly attached to the culture carrier. If the adhesion strength is not good, the cells will fall off and affect the culture yield. The problem of adhesion restricts the selection and application range of algae species and carrier materials in the breeding process. In order ...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N5/04C12R1/89
CPCC12N1/20C12N1/12
Inventor 石蕾程小雪陈昱王琳崔春莉白雪梅
Owner ENN SCI & TECH DEV
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