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Method for determining content of sesamin in sesame

A technology for sesamin and content, applied in directions such as measuring devices, instruments, scientific instruments, etc., can solve the problems such as large consumption of sesame oil samples and solvents, large error in detection results, long required time, etc., and achieve shortening of detection requirements. time, improved extraction rates, simplified steps and effects

Inactive Publication Date: 2015-07-15
HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
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Problems solved by technology

[0003] At present, chromatography methods are mainly used for the determination of sesamolin content, such as thin layer chromatography (TLC), gas chromatography (GC), high performance liquid chromatography (HPLC), etc., but the detection objects of these methods are all sesame oil ( For example, the Chinese patent document CN101602768), the sesame oil needs to be subjected to complex pretreatment before detection, the steps in the detection process are cumbersome, the time required is long, the consumption of sesame oil samples and solvents is large, and the detection results have large errors

Method used

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  • Method for determining content of sesamin in sesame
  • Method for determining content of sesamin in sesame
  • Method for determining content of sesamin in sesame

Examples

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Embodiment 1

[0030] A method for measuring sesamolin content in sesame, comprising the following steps:

[0031] (1) To make a standard working curve: use methanol solutions of sesamolin standard substances with gradient concentrations of 200, 100, 50, 25, 12.5, and 6.25 μg / mL, and use anhydrous methanol as a blank control to measure the absorbance at a wavelength of 287 nm , with the concentration as the abscissa and the absorbance as the ordinate, draw a standard working curve, and the corresponding fitting equation is y=457.729323x-3.3962015, where y is the peak area, and x is the sesamolin content;

[0032] (2) Sample treatment: crush the sesame sample, pass it through a 0.35 μm sieve, weigh 0.2000 g of the obtained powder, add 7 mL of absolute ethanol, and use ultrasonic waves (ultrasonic power: 80 W) and oscillator for 25 min intermittently at 60 ° C (Sonication for 5 minutes, shaking for 1 minute, and so on cycle), then, centrifuge at 5000rpm for 2min, take the supernatant in a syri...

Embodiment 2

[0035] It is basically the same as in Example 1, except that in step (2), anhydrous ethanol 30 times the weight of the sesame powder is added, and it is treated intermittently with ultrasonic wave and oscillator for 25 minutes.

Embodiment 3

[0037] It is basically the same as in Example 1, except that in step (2), anhydrous ethanol 40 times the weight of the sesame powder is added, and it is treated intermittently with ultrasonic wave and oscillator for 30 minutes.

[0038] Unless otherwise specified, the instruments and equipment involved in the above examples are conventional instruments and equipment; the involved industrial raw materials are commercially available conventional industrial raw materials unless otherwise specified.

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Abstract

Belonging to the technical field of plant detection, the invention specifically relates to a method for determining the content of sesamin in sesame. The method includes the steps of: (1) drawing a standard work curve at a 287nm wavelength site; (2) crushing a sesame sample and then conducting specific treatment; (3) performing HPLC determination on the sample, and carrying out lookup or calculation according to the prepared standard work curve, thus obtaining the content of sesamin in the sesame sample. The determination method provided by the invention is simple, fast and efficient, has high sensitivity, can maximumly extract sesamin in the sample to conduct determination so as to be closer to a true value, and can be carried out on a large scale.

Description

technical field [0001] The invention belongs to the technical field of plant detection, and in particular relates to a method for measuring the content of sesamolin in sesame. Background technique [0002] Sesame is one of the important oil crops in my country and has high application value. Sesamin belongs to lignan, and its content in sesame oil is 0.2% to 0.6%. a physiological activity. my country is rich in sesame resources and has great potential for development. Sesame can be planted in a wide range of areas in my country, but the quality of sesame produced in different areas is uneven, and the content of sesamolin is very different. At present, the national standard classifies sesame according to different indicators, but does not specify the content of sesamolin. The prices of different grades of sesame vary greatly. In view of the high added value of sesamolin, it is of practical significance to include the content of sesamolin in the standard of classifying se...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 许兰杰梁慧珍余永亮杨红旗董薇牛永光张收良聂占胜李红莲
Owner HENAN SESAME RES CENT HENAN ACADEMY OF AGRI SCI
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