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Phomopsis viticola and phomopsis obscurans resisting applications of dahurian angelica root active components

An active ingredient, Phomopsis technology, applied in the application field of Angelica dahurica active ingredients against Phomopsis viticola and Phomopsis obscurus, can solve complex extraction process, poisoning, natural plant varieties and few resources and other problems, to achieve the effect of simple extraction process, wide distribution and easy cultivation

Inactive Publication Date: 2015-07-22
HUNAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the inhibitors against Phomopsis viticosa and Phomopsis dusk are chemical insecticides, which are widely used for a long time and are easy to pollute the air, waters, and soil, posing threats to human and animal health, and even poisoned
However, the extraction of antibacterial agents from natural plants has complex extraction processes, few natural plant varieties and resources, and does not have wide applicability.

Method used

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  • Phomopsis viticola and phomopsis obscurans resisting applications of dahurian angelica root active components
  • Phomopsis viticola and phomopsis obscurans resisting applications of dahurian angelica root active components
  • Phomopsis viticola and phomopsis obscurans resisting applications of dahurian angelica root active components

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Isolation and identification of active ingredients of Angelica dahurica

[0035] 1.1 Experimental materials, reagents and instruments

[0036] 1.1.1 Medicinal materials

[0037] Angelica dahurica is purchased from the Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine, produced in Sichuan, a GMP certified enterprise, produced by Hunan Songlingtang Chinese Herbal Pieces Co., Ltd., and the production batch number is 120401. Angelica dahurica decoction pieces 3kg, reserved sample 100g, preserved in the National Medicine Innovation and Development Laboratory of Traditional Chinese Medicine (No. 201204628-1). The medicinal material is identified as the root of Angelica dahurica.

[0038] 1.1.2 Reagents

[0039] Silicone G, GF 254 , Silicone H (Qingdao Ocean Chemical Co., Ltd.);

[0040] 95% ethanol is chemically pure; distilled water: self-made; petroleum ether, dichloromethane, chloroform, ethyl acetate, ethanol, methanol, acetonitrile (anal...

Embodiment 2

[0071] Example 2 Determination of the Active Components of Angelica dahurica against Phomopsis viticola and Phomopsis obscurus

[0072] A 96-well plate microtiter assay was used to determine the inhibitory effect of individual compounds against Phomopsis obscurans Phomopsis viticola and to compare their activity with fungicides with known mechanisms of action.

[0073] Three different concentrations of 0.3, 3.0 and 30.0 μM were arrayed against Phomopsis viticola and Phomopsis dusk in 96-well plates in different columns, and each experiment was replicated once. At a wavelength of 620nm, the growth of fungi was evaluated by measuring the absorbance of each small well in the 96-well plate at time points of 0, 24, 48, and 72 hours, and the data was recorded until 120 hours. The difference in spore germination and mycelium growth in 96-well plates shows the effect of specific concentrations of monomeric compounds on fungal growth, thereby obtaining fungal inhibition data.

[0074]...

Embodiment 3

[0076] Embodiment 3 anti-phymopsis viticola medicament

[0077] Preparation of the anti-Phomobacterium viticola medicament: take xanthoxylin, bergamotide, allo-imperatorin and xanthoxylin to prepare the anti-Phomobacterium viticola medicament, and the auxiliary material is water. The concentrations of xanthoxylin, berganolide, allo-imperatorin and xanthoxylin in the drug were 0.1 μM, 0.3 μM, 0.1 μM and 0.2 μM, respectively.

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Abstract

Phomopsis viticola and phomopsis obscurans resisting applications of dahurian angelica root active components (which are xanthoxylin, bergapten, alloimperatorin and xanthotoxol) are provided. Phomopsis viticola resisting activity and phomopsis obscurans resisting activity of the bergapten are the highest which are respectively 99.8% and 88.3%.

Description

technical field [0001] The invention relates to plant active components, in particular to the application of the active components of Angelica dahurica in resisting Phomopsis viticola and Phomopsis obscurus. Background technique [0002] Increasing chemical resistance of plant pests and pathogens has led to increased use of pesticides, forcing us to find new fungicides and insecticides for agricultural use. The EPA and EFSA have identified azole fungicides as problematic because of their effects on the endocrine system, as well as liver toxicity and other serious problems. Therefore, looking for environmentally friendly pesticides that are safer and less toxic to mammals has become an important research content for agricultural development today. There is great potential to discover and develop new antibacterial and biopesticide compounds from natural plants, and natural plants have become an important source of new drug research and development today. [0003] Angelica da...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01N43/90A01P3/00C07D311/16C07D493/04
Inventor 王炜江星明李顺祥李斌谢谦黄建华彭彩云
Owner HUNAN UNIV OF CHINESE MEDICINE