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Strontium chondroitin sulfate and its preparation method

A technology of chondroitin sulfate and sodium chondroitin sulfate, applied in the biological field, can solve the problems of poor prevention effect of osteoporosis and osteoarthritis, petechiae and hematoma, etc.

Active Publication Date: 2018-03-09
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The traditional methods for extracting chondroitin sulfate mainly include alkaline extraction, enzymatic hydrolysis, alkaline extraction, ultrasonic-assisted method and acetic acid extraction, etc. The chondroitin sulfate prepared by these traditional methods mainly exists in the form of sodium chondroitin sulfate, and chondroitin sulfate The affinity for calcium is better than that of sodium. After use, it is easy to cause calcium accumulation in blood vessels and capillaries, resulting in petechiae and hematoma. It is less effective in preventing osteoporosis and osteoarthritis.

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preparation example Construction

[0035] Such as figure 1 As shown, the preparation method of strontium chondroitin sulfate in one embodiment can be used to prepare the above-mentioned strontium chondroitin sulfate. The preparation method includes the following steps:

[0036] Step S110: Pass the aqueous solution of sodium chondroitin sulfate through a cation exchange resin column, start collecting the effluent when the pH of the effluent changes from neutral to 4.5-5, stop collecting until the pH of the effluent becomes 6.5-7.

[0037] Among them, sodium chondroitin sulfate can be purchased from the market; it can also be prepared by oneself.

[0038] Wherein, the preparation steps of sodium chondroitin sulfate are as follows: degrease the cartilage and pulverize it to obtain cartilage powder; mix the cartilage powder with deionized water, then add an alkalizing agent, and stir at 30°C to 40°C until the cartilage powder dissolves to obtain Cartilage solution; wherein, the mass ratio of alkalizing agent to ca...

Embodiment 1

[0056] The preparation steps of strontium chondroitin sulfate of the present embodiment are as follows:

[0057] (1) Preparation of sodium chondroitin sulfate: animal cartilage was boiled in deionized water at 80°C for 3 hours, after oil was removed, it was then boiled in deionized water at 80°C for 4 hours, cooled and then dried.

[0058] After the cartilage is degreased, it is pulverized to obtain cartilage powder. Put the cartilage powder in a reaction tank, add 5 times the deionized water of the cartilage and mix, then add sodium hydroxide according to the mass ratio of sodium hydroxide to cartilage is 0.01:1, and then Stir at 40°C until the cartilage powder dissolves to obtain a cartilage solution; use an aqueous solution of hydrochloric acid with a concentration of 3 mol / L to adjust the pH value of the cartilage solution to 8, and add trypsin according to the mass ratio of trypsin to cartilage of 0.0045:1, and add trypsin at 40 ℃ enzymatic hydrolysis for 4 hours, then wa...

Embodiment 2

[0064] The preparation steps of strontium chondroitin sulfate of the present embodiment are as follows:

[0065] (1) Preparation of sodium chondroitin sulfate: animal cartilage was boiled in deionized water at 100°C for 2 hours, after oil was removed, then boiled in deionized water at 100°C for 2 hours, cooled and then dried.

[0066] After the cartilage is degreased, it is pulverized to obtain cartilage powder. The cartilage powder is placed in a reaction tank, mixed with deionized water 30 times the amount of cartilage, and then sodium bicarbonate is added according to the mass ratio of sodium bicarbonate to cartilage of 0.5:1. Stir at 30°C until the cartilage powder dissolves to obtain a cartilage solution; use an aqueous solution of hydrochloric acid with a concentration of 4mol / L to adjust the pH value of the cartilage solution to 9, and add trypsin according to the mass ratio of trypsin to cartilage as 0.0055:1, and add trypsin at 50 ℃ enzymatic hydrolysis for 5 hours, t...

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Abstract

The invention relates to strontium chondroitin sulfate and a preparation method thereof. The molecular formula of the strontium chondroitin sulfate is H2O(C14H19NSrO14S)x, wherein x is an integer of 60-100. According to the strontium chondroitin sulfate, the problems of petechiae and hematoma caused by calcium deposition of blood vessel parts and blood capillary parts can be avoided, and osteoporosis and osteoarthritis can be better treated in an aid way.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to strontium chondroitin sulfate and a preparation method thereof. Background technique [0002] Chondroitin sulfate is a mucopolysaccharide extracted from animal cartilage, mainly composed of D-glucuronic acid and N-acetyl-D-galactosamine. It has a protective effect on bones, can prevent bones from becoming hard and brittle, prevent further calcium hardening, maintain a certain degree of flexibility in bones, help the recovery of bone hyperplasia, and play an important role in bone repair and prevention of joint diseases. effect. [0003] The traditional methods for extracting chondroitin sulfate mainly include alkaline extraction, enzymatic hydrolysis, alkaline extraction, ultrasonic-assisted method and acetic acid extraction, etc. The chondroitin sulfate prepared by these traditional methods mainly exists in the form of sodium chondroitin sulfate, and chondroitin sulfate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
Inventor 马粉波唐斌温春毅李慧丽葛永梅
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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