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Enterococcus facium strain and application thereof

A technology of Enterococcus faecium and bacterial strains, applied in the field of microbial strain breeding

Active Publication Date: 2015-07-22
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the use of Enterococcus faecium to ferment traditional Chinese medicines, especially Codonopsis pilosula and Astragalus membranaceus, there are no domestic and foreign literature reports.

Method used

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  • Enterococcus facium strain and application thereof
  • Enterococcus facium strain and application thereof
  • Enterococcus facium strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Enterococcus faecium ( Enterococcus facium ) Obtaining of C8GF20-06 strain CGMCC No.8476:

[0017] 1. Isolation and purification of Enterococcus faecium C8GF20 mixed strains

[0018] In the previous research, the inventor's research group isolated the mixed bacteria C8GF20 from the cecum of healthy native chickens that had not used antibiotics, and it has been disclosed in the following ways: 1. Zhang Kai, Yang Zhiqiang, etc., chicken intestinal bacteria fermented Codonopsis pilosula Experimental research on transforming polysaccharides, Journal of Chinese Veterinary Medicine, 2008; 2. Li Jianxi, Research on biotransformation technology of Chinese veterinary medicines - the effect of probiotic fermentation on the extraction of polysaccharides from Radix Astragali Codonopsis, Chinese Academy of Agricultural Sciences, doctoral dissertation, 2012. The above-mentioned personnel guarantee to provide the isolated mixed bacteria C8GF20 to the public within 20 years from the d...

Embodiment 2

[0048] Test of using Enterococcus faecium CGMCC No.8476 of the present invention to ferment Astragalus root and Radix Codonopsis:

[0049] (1) Fermented Dangshen

[0050] Enterococcus faecium CGMCC No.8476 was inserted into MRS broth, cultured on an anaerobic shaker at 120r / min, 37°C; The formula of the medium is: corn flour 25.79 g; nitrogen source 0.6 g; carrot powder 2.28 g; beef extract 1.56 g; inorganic salt K 2 HPO 4 -KH 2 PO 4 Codonopsis 2.18g; Codonopsis 120-150g; 1000mL distilled water), placed in an anaerobic environment at 37°C, with an initial pH of 6.4-7.4, and fermented at 120r / min for 48h; the fermentation broth was vacuum freeze-dried to obtain fermented Codonopsis pilosula. After testing, the polysaccharide content in the fermented Codonopsis pilosula was 196.76±14.16mg / g.

[0051] The applicant has selected the formula of the fermentation medium and the fermentation conditions through experiments. After testing, when the above fermentation medium and fe...

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Abstract

The invention provides an enterococcus facium C8GF20-06 strain. The collection No. of the enterococcus facium C8GF20-06 strain is CGMCC No.8476. One enterococcus facium strain CGMCC No.8476 is selected and cultured on the basis of a C8GF20 strain. The strain is gram-positive bacterium, belongs to enterococcus, has the characteristic of high polysaccharide conversion capacity, can be used for fermentation of traditional Chinese medicines such as radix codonopsis, astragalus membranaceus and the like and has the advantages of increase of an extraction ratio of effective content and active substances in medicines, improvement of efficacy, reduction of waste of traditional Chinese medicine resources and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial strain breeding, and in particular relates to a chicken-derived Enterococcus faecium strain and its application. Background technique [0002] Traditional Chinese medicine fermentation technology refers to the use of the powerful biotransformation ability of microorganisms existing in nature to transform the active ingredients of medicinal materials, reduce the toxicity of ingredients, and extract and separate the active ingredients. Microbial fermentation of traditional Chinese medicine mainly has the following characteristics and advantages: mild reaction, protective activity; improved drug efficacy, reduced toxicity; modified structure, discovery of new drugs; stable quality, conducive to modern times; rich strains, controllable direction; saving drug sources, increasing benefits . Excellent microbial strains are the foundation and key of traditional Chinese medicine fermentation technology....

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K36/481A61K36/344C12R1/01
CPCA61K36/344A61K36/481C12N1/20C12N1/205C12R2001/01
Inventor 李建喜张景艳杨志强王学智张凯秦哲王旭荣王磊孔晓军孟嘉仁
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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