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A method and device for micron-scale zonal sampling suitable for capillary

A sampling device and capillary technology, applied in the field of separation and analysis in analytical chemistry, can solve the problems of increasing sample loss, increasing the difficulty of analyzing precious samples, prolonging the analysis process, etc.

Active Publication Date: 2017-03-22
INST OF CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This pretreatment will not only prolong the entire analysis process, but also increase the loss of samples during the processing process, which intensifies the difficulty of analyzing some precious samples

Method used

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  • A method and device for micron-scale zonal sampling suitable for capillary
  • A method and device for micron-scale zonal sampling suitable for capillary
  • A method and device for micron-scale zonal sampling suitable for capillary

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, sampling device

[0046] The structure of the sampling device provided by the invention is as figure 1 As shown, it consists of a quartz capillary 1, a detachable sample loading plate 2, a displacement control mechanism 3, a capillary cleaning mechanism that is a syringe 5 and a capillary holder 4;

[0047] Wherein, the inner diameter of the quartz capillary 1 is 50 μm, the outer diameter is 375 μm, and the length is 5 cm; one end of the capillary is a sharpened sampling end, and is hydrophobized with octadecyl silicon chloride; the quartz capillary points vertically to the sample plate, The other end is connected to the capillary cleaning mechanism, that is, the syringe;

[0048] The sample adding plate 2 is a glass sheet, which is placed on the displacement control mechanism, and a groove of 1 cm × 1 cm × 200 μm is etched with HF on it as a sample adding slot; the number of sample adding slots is three;

[0049] The displacement control mechanism 3 is ...

Embodiment 2

[0051] Embodiment 2, sampling and effect of FITC liquid sample

[0052] 1) Using the sample injection device of Example 1, the inner wall of the capillary is cleaned with the capillary cleaning mechanism, that is, the syringe 5, and then the buffer capable of dissolving the sample is sucked or pressed into the capillary until the entire tube is filled.

[0053] The sample plate 2 was cleaned with Piranha solution and treated with air plasma to make it hydrophilic.

[0054] 2) Configure the FITC liquid sample as follows:

[0055] Use 5 mM borax aqueous solution with a pH value of 9.2 as a solvent, dissolve FITC in the solvent to a concentration of 0.1 mM; take 1 μL of the above solution as a FITC liquid sample;

[0056] 3) Drop the FITC liquid sample obtained in step 2) into a 1 cm × 1 cm × 200 μm sampling tank on the sample loading plate 2, spread it out, and obtain a sample liquid film with a thickness of 10 μm, such as figure 2 (upper left);

[0057] Fix the sample addin...

Embodiment 3

[0059] Example 3, Sampling and effect of FITC solid sample

[0060] Utilize the sampling device of embodiment 1, the FITC liquid sample used in embodiment 2 is dripped in the sampling tank of sample loading plate 2, after spreading out, let it volatilize and dry naturally (such as figure 2 Shown in the lower left), obtain FITC solid sample;

[0061] Afterwards, the sample loading plate 2 was placed on the displacement control mechanism 3 for sample injection, and the contact time was 0.2s.

[0062] Using a fluorescence microscope to observe the sample injection zone, it can be known that the length of the sample injection zone is 8 μm, as figure 2 Shown below right. The length of the sampling zone can be adjusted by the length of contact time between the capillary and the solid sample surface. It can be seen from the figure that the sample injection of FITC solid samples can be realized by using the sample injection device of Example 1.

[0063] The same can be done by c...

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Abstract

The invention discloses a micron-sized zone sampling method and device suitable for a capillary tube. The sampling device comprises a capillary tube, sampling plates, a displacement control mechanism and a capillary tube cleaning mechanism, wherein one end of the capillary tube is the sampling end; the sampling end is polished to be flat or sharp, and is perpendicularly pointed to the sampling plates; the other end of the capillary tube is connected with the capillary tube cleaning mechanism; the sampling plates are placed on the displacement control mechanism; the displacement control mechanism is used for controlling the sampling plates to horizontally move in the long axis or short axis direction of the capillary tube. The sampling method utilizes the in situ dissolution and diffusion principle to transfer sample components, can be applied to in situ direct sampling of the liquid sample, the solid sample and the transition state sample of the solid sample, and can reduce the sample pretreatment steps; the sampling zone is micron-sized, and the sampling method is urgently needed by the short capillary tube separation technology; the sampling is simple and convenient to control, and the device is easy to design and manufacture.

Description

technical field [0001] The invention belongs to the field of separation and analysis in analytical chemistry, and relates to a micron-level zone sampling method and device suitable for capillary tubes. Background technique [0002] Separations and other analyzes can be performed using capillaries. However, when doing ultra-trace analysis, ultra-short injection technology is required. The most influential capillary analysis method is capillary electroseparation (CES), including capillary electrophoresis and capillary electrochromatography. As a high-efficiency micro-volume liquid phase separation analysis method with capillary as the separation channel, CES has already shown great advantages in the separation and analysis of major biomolecules such as nucleic acids, sugars, and proteins, but in the process of advancing to the analysis of complex samples However, they are facing new and huge challenges. Among them, how to further speed up the separation and effectively reduc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/16
Inventor 陈义胡灿
Owner INST OF CHEM CHINESE ACAD OF SCI
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