Method for measuring contribution rate of soil adsorbed amino acid to plant nitrogen nutrition
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An amino acid, adsorption state technology, used in soil testing, material testing, etc.
Inactive Publication Date: 2015-07-22
CHINA NAT RICE RES INST
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In conclusion, there is still a great deal of controversy about the accurate evaluation of the bioavailability of amino acids
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Embodiment 1
[0027] As shown in 1, firstly, the paddy field soil A in Xiaoshan, Zhejiang Province (10m above sea level, 30o3' 59.2" N, 120o15' 31.4" E) was collected, air-dried and ground, passed through a 20-mesh sieve for later use; 5 grams of soil was weighed, Add 25 ml of 0.5 mol / L potassium sulfate solution and 2 drops of chloroform, shake (150 rpm, 60 min), centrifuge (4000 rpm, 10 min), filter and discard the eluent, and repeat this washing step 5 times to elute the amino acids adsorbed on the soil surface; the soil after rinsing was sterilized by high-temperature and high-pressure steam in a 121°C autoclave for 30 minutes, and this sterilization step was repeated 3 times to completely inactivate the microorganisms in the soil;
[0028] Then, add 25ml of a series of 15 N-labeled glycine solution for adsorption test (0, 5, 10, 15, 30, 60, 120, 240, 480, 700, 800, 1000, 1200 mg / L), the glycine solution was prepared by sterile water, and The 0.22μm microporous membrane filtration achi...
Embodiment 2
[0040] Firstly, the alpine paddy field soil B in Xianju County, Zhejiang Province (700m above sea level, 28o56' 39.6"N, 120o46' 39.6"E) was collected, air-dried and ground, passed through a 20-mesh sieve for later use; 5 grams of soil was weighed, and 25 ml 0.5 mol / L potassium sulfate solution and 2 drops of chloroform, shake (150 rpm, 60 min), centrifuge (4000 rpm, 10 min), filter and discard the eluent, repeat this washing step 5 times until Elute the amino acids adsorbed on the surface of the soil; sterilize the washed soil by high-temperature and high-pressure steam for 30 minutes in an autoclave at 121°C, and repeat this sterilization step 3 times to completely inactivate the microorganisms in the soil;
[0041] Then, a series of 15 N-labeled glycine solution for adsorption test (0, 5, 10, 15, 30, 60, 120, 240, 480, 700, 800, 1000, 1200 mg / L), the glycine solution was prepared by sterile water, and The 0.22μm microporous membrane filtration achieves a sterile effect. Af...
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Abstract
The invention provides a method for measuring a contribution rate of soil adsorbed amino acid to plant nitrogen nutrition, and belongs to the technical field of biogeochemistry. The method comprises the following steps: airing and grinding collected soil, performing sieving, and using the processed soil for later use; performing repeated leaching on soil with a strong potassium sulfate salt solution; placing leached soil in an autoclave to carry out high temperature and high pressure steam sterilization treatment; adding 15N-labeled glycine solutions with different concentrations into the soil subjected to sterilization treatment to carry out adsorption tests; performing oscillation, centrifugation and filtration, and calculating a glycine adsorption curve in the soil according to the glycine concentration difference in the solution before and after adsorption; according to the glycine adsorption curve, selecting soils at the adsorption saturation point and the adsorption half saturation point of the curve; performing cultivation tests on paddy rice seedlings in a sterile culture room, harvesting paddy rice after 21 days, and calculating the contribution rate of absorption of amino nitrogen and amino acid to paddy rice nitrogen nutrition. The method has a very important function of revealing fertility of soil organic nitrogen and nitrogen cycle of an ecological system.
Description
technical field [0001] The invention belongs to the technical field of biogeochemistry, and in particular relates to a method for measuring the contribution rate of soil adsorbed amino acids to plant nitrogen nutrition. Background technique [0002] Nitrogen is a macronutrient element for the biosynthesis of amino acids, proteins and other nitrogen-containing organic substances, and plays an extremely important role in the life activities of plants. As an important form of soil organic nitrogen, amino acid has gradually attracted people's attention recently. Amino acids in soil are divided into free and adsorbed, the concentration of the former is very low, only 1-158 μmol / L; while the content of amino acids adsorbed on the solid surface of soil is much higher than that of free amino acids, accounting for about 88%-92 of the total amount of amino acids in soil. %. Compared with inorganic nitrogen, although the content of amino acid nitrogen is very low, its short half-life...
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