Method for detecting benzoic acid in food

A technology of benzoic acid and food, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of no sterilization, antibacterial effect, and not very good sterilization effect, and achieve the effect of obvious peak and accurate measurement

Active Publication Date: 2015-07-29
WUZHOU INST OF PROD QUALITY INSPECTION
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Problems solved by technology

Let me briefly introduce sodium benzoate: sodium benzoate is mostly white particles, odorless or slightly benzoin smell, slightly sweet, astringent; easily soluble in water (room temperature) at about 53.0g/100ml, pH at about...

Method used

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Embodiment 1

[0022] A method for detecting benzoic acid in food, comprising the steps of:

[0023] Step 1: pulverize or grind the food sample with a medical pulverizer;

[0024] Step 2: Take 1g of the food sample treated in step 1, add 0.5ml of NaOH solution with a concentration of 1mol / L, add water to make up to 20ml;

[0025] Step 3: Mix the solution after constant volume with a vortex mixer, put it into a 100ml centrifuge tube and centrifuge, and take the supernatant;

[0026] Step 4: Add 10ml of ethyl acetate and 10ml of n-hexane to the supernatant, and separate the oil phase and the water phase after stirring for a period of time;

[0027] Step 5: take the water phase and carry out high-performance liquid chromatography for determination; the parameters of the liquid chromatography are set as follows:

[0028] Chromatographic column: C18 column, 150mm*3.9mmID

[0029] Column temperature: room temperature

[0030] Mobile phase: 0.05% phosphoric acid aqueous solution, ethanol mixed ...

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Abstract

The invention discloses a method for detecting benzoic acid in food. The method comprises the following steps of 1) crushing or grinding a food sample by utilization of a medical crusher; 2) taking 1 g of the food sample processed in the step 1, adding 0.5 ml of NaOH solution withconcentration of 1 mol/L, and diluting with water to a volume of 20 ml; 3) mixing the diluted solution through a vortex mixer, putting the mixed solution into a centrifugal tube with a volume of 100 ml for centrifugation, and taking supernate; 4) adding 10 ml of ethyl acetate and 10 ml of normal hexane into the supernate, and separating an oil phase from a water phase after stirring the supernate for a period of time; 5) taking the water phase to perform high-performance liquid chromatographic determination. The invention aims to provide the method for detecting the benzoic acid in the food, which has the advantage of accuracy in measurement.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a method for detecting benzoic acid in food. Background technique [0002] Benzoic acids include benzoic acid and sodium benzoate; benzoic acid is also called benzoic acid, so sodium benzoate is also called sodium benzoate. Benzoic acid is insoluble in water at normal temperature, slightly volatile in air (especially hot air), hygroscopic, about 0.34g / 100ml at normal temperature; but soluble in hot water; also soluble in ethanol, chloroform and non-volatile Oil. And sodium benzoate uses sodium benzoate in China; the properties and antiseptic properties of benzoic acid and sodium benzoate are similar. Let me briefly introduce sodium benzoate: sodium benzoate is mostly white particles, odorless or slightly benzoin smell, slightly sweet, astringent; easily soluble in water (room temperature) at about 53.0g / 100ml, pH at about 8; sodium benzoate It is also an acidic preservative, and ...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
Inventor 朱海军梁超雄吴婕蔡宇春谭略
Owner WUZHOU INST OF PROD QUALITY INSPECTION
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