A calibration system for RDSS (Radio Determination Satellite Service) channel zero value comprises a spread spectrum signal source, a microwave signal source, a frequency converter, a power divider, a double-channel digital oscilloscope, a first attenuator, a second attenuator, a control and processing computer and a vector network analysis instrument, wherein the spread spectrum signal source generates simulated RDSS spread spectrum signals; spread spectrum signal source outcoming signals are subjected to frequency conversion by the microwave signal source and the frequency converter so as to generate frequency points suitable for RDSS service channel input; a high-sampling rate digital oscilloscope is used for synchronously sampling the input/output signals of the RDSS service channel; and the control and processing computer is used for completing initialization of the oscilloscope, acquisition and loading of the oscilloscope channel data, orthogonalization of the channel data, carrier rotation, circulating autocorrelation, peak value searching, and zero value calculation and processing, so as to eventually obtain the absolute time delay of the RDSS service channel. According to the system provided by the invention, the signals consistent with the RDSS service system are adopted to serve as the reference, the calibration zero value can reflect the real conditions and the calibration precision is high.