Method for producing sophorose from sophorolipids

A technology of sophorolipids and sophorose, applied in the field of expression solutions, can solve the problem that sophorose cannot be used on an industrial scale

Inactive Publication Date: 2015-07-29
INST FR DU PETROLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Unfortunately, the high price of sophorose means that it is still not available on an industrial scale

Method used

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  • Method for producing sophorose from sophorolipids
  • Method for producing sophorose from sophorolipids
  • Method for producing sophorose from sophorolipids

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Embodiment Construction

[0024] The present invention describes the conditions for carrying out the acid hydrolysis with the aim of producing sophorose and avoiding the complete hydrolysis of the sophorolipid which would lead to the release of two glucose units per molecule of the sophorolipid.

[0025] Under the conditions described below, hydrolysis can be used to preferentially break the glycosidic bond between the sophorose and the aliphatic chain, which leads to the release of the sophorose. However inevitably and to a greater or lesser extent depending on the operating conditions, the released sophorose may itself also be hydrolyzed into two glucose units.

[0026] In practice, an aqueous solution of sophorolipids is first provided. The sophorolipid concentration of the aqueous solution is usually 2-800 g / L, preferably 100-400 g / L. Preferably, the sophorolipids will be used in the acid form and not in the lactone form. This acid form can be obtained by optimizing the fermentation conditions du...

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Abstract

The present invention concerns a method for producing sophorose from a sophorolipid which involves carrying out a step of hydrolysis of the sophorolipid in an aqueous medium in the presence of an acid compound.

Description

[0001] The present invention relates to a method for producing sophorose by acid hydrolysis of sophorolipids. The present invention also relates to the use of the hydrolyzate obtained using the method of the present invention as a solution for inducing the expression of a gene encoding a cellulase. Finally, the invention also relates to a method for purifying the sophorose of the hydrolyzate. Background technique [0002] Cellulases suitable for the hydrolysis of polysaccharides allow the microorganisms producing them to utilize cellulose and hemicellulose (the main components of plants) as a carbon source by hydrolyzing these polymers into simple sugars (glucose, xylose, etc.). [0003] These enzymes are currently used in many fields, such as detergents, the textile industry or the food industry, and in processes aimed at the biochemical value addition of lignocellulosic biomass (e.g. for the production of second-generation ethanol biofuels) . Currently, Trichoderma reesei ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/00C07H3/04C12N1/38C12N9/42
CPCC07H3/04C07H1/00C12P19/12C07H1/06
Inventor E·茹尔迪埃F·本沙巴纳
Owner INST FR DU PETROLE
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