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Antibacterial peptide PLC13 and application thereof to inhibition of peri-implantitis bacteria

A PLC13 and antimicrobial peptide technology, applied to the antimicrobial peptide PLC13 and its application field in inhibiting peri-implantitis bacteria, can solve the problems of difficult synthesis, high synthesis cost, long peptide chain, etc., and achieves low hemolysis and production. The effect of low cost and short peptide chain

Active Publication Date: 2015-08-05
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

SMAP29, LL-37, HBD2, HBD3, Cecropin B, etc. reported in the existing literature all show strong antibacterial effects, but at the same time, these antibacterial peptides have different peptide chain lengths, difficulty in synthesis, and high cost of synthesis. degree of cytotoxicity

Method used

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  • Antibacterial peptide PLC13 and application thereof to inhibition of peri-implantitis bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] Cytotoxicity assay of antimicrobial peptide PLC13 on human erythrocytes.

[0038] Experimental method: Take 1.5ml EP tube and add 0.5ml of antimicrobial peptide PLC13 and 10% erythrocyte suspension respectively, and the corresponding final concentrations of antimicrobial peptides are 100, 50, 25, 12.5, 6.2, 3.1 μg / ml respectively; positive control group is set , take a 1.5ml EP tube and add 0.2% Tritonx-100 diluted with normal saline and 0.5ml each of 10% red blood cell suspension; set up a negative control group, take a 1.5ml EP tube and add 0.5ml each of normal saline and 10% red blood cell suspension. Add sample and mix well, incubate in 37°C incubator for 1 hour, centrifuge, absorb supernatant from each tube and add to 96-well plate, add 200 μl of liquid to each well, set three parallel wells for each group of samples. Measure the optical density value at 540nm of the microplate reader, and calculate the hemolysis: hemolysis = (optical density value of the antimicro...

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Abstract

The invention belongs to the technical field of biology, and specifically discloses an antibacterial peptide PLC13 and application thereof to inhibition of peri-implantitis bacteria. The sequence of the antibacterial peptide PLC13 is shown as SEQ ID NO: 1; one end of a peptide chain of the antibacterial peptide is connected with acetate, and the other end of the peptide chain of the antibacterial peptide is connected with an amino group. The antibacterial peptide PLC13 is discovered to have obvious antibacterial activity to the peri-implantitis bacteria, is safe and nontoxic to human bodies, meanwhile has the characteristics of short peptide chain, easiness in synthesis, low cost and the like, and can be effectively applied to inhibiting the infection of oral cavity by the peri-implantitis bacteria, particularly the infection of fusobacterium nucleatum and staphylococcus aureus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an antibacterial peptide PLC13 and its application in inhibiting peri-implantitis bacteria. Background technique [0002] Implant dentures have good stability and a high long-term success rate. However, with the increase in the number of patients receiving dental implant treatment, the incidence of peri-implantitis also increases rapidly. The key to preventing peri-implantitis is infection control. In addition to strict aseptic operation during the operation and teaching patients to maintain oral hygiene, it is even more important to prevent it with antibacterial drugs before inflammation occurs or when there is mild inflammation. treat. This is especially important in patients with a history of chronic periodontitis. Studies have shown that the probability of peri-implantitis in patients with periodontitis after implant treatment is significantly higher than that of pat...

Claims

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Application Information

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IPC IPC(8): C07K7/08A01N47/44A01P1/00
Inventor 金丰良许小霞余静高延富欧阳莉娜张玉清鞠雯燕杨涛刘长虹
Owner SOUTH CHINA AGRI UNIV
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