A method for determining the content of cathinone and 4-methylmethcathinone in a sample by using high performance liquid chromatography
A technology of methyl methcathinone and high-performance liquid chromatography, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of reduced criminal investigation work efficiency, low detection efficiency, and long detection time, so as to shorten the detection time, Improve the degree of separation and ensure the effect of effective separation
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Embodiment 1
[0031] The present invention adopts the method for measuring cathinone and 4-methylmethcathinone content in the sample by high performance liquid chromatography, comprising the steps of:
[0032] (1) Preparation of working solution; take cathinone and 4-methyl methcathinone standard stock solutions respectively, and dilute successively with solutions of internal standard substances with a mass concentration of 0.1 mg / mL to form cathinone and 4-methyl methcathinone respectively. Two series of standard solutions with methyl methcathinone mass concentrations of 0.5, 0.1, 0.05, 0.01, 0.005, 0.001, and 0.0005 mg / mL, and the concentration of the internal standard solution was kept at 0.01 mg / mL.
[0033] (2) Preparation of sample solution: Weigh about 45 mg of sample materials containing cathinone and 4-methylmethcathinone respectively (the sample inspection materials in this embodiment use cathinone standard and 4-methylmethcathinone respectively). Prepared after mixing methcathino...
Embodiment 2
[0040] The present invention adopts the method for measuring cathinone and 4-methylmethcathinone content in the sample by high performance liquid chromatography, comprising the steps of:
[0041] (1) Preparation of working solution: Take cathinone and 4-methylmethcathinone standard stock solutions respectively, and dilute them in sequence to mass concentrations of 0.5, 0.1, 0.05, 0.01, 0.005, 0.001, 0.0005mg / mL Two series of standard solutions.
[0042](2) Preparation of sample solution: Weigh about 45 mg of sample materials containing cathinone and 4-methylmethcathinone respectively (the sample inspection materials in this embodiment use cathinone standard and 4-methylmethcathinone respectively). Prepared after mixing methcathinone with other substances, the content of cathinone in sample 1 is 49.86wt%, and the content of 4-methyl methcathinone in sample 2 is 50.42wt%), respectively with a bottle-top pipette Add 20mL mobile phase for extraction, shake for 10 minutes, and cen...
Embodiment 3
[0049] The difference between this example and Example 1 is that the first sample used is blood with a cathinone content of 49.86wt%, that is, blank blood is taken, and a standard cathinone is added; the second sample used is 4-methylmethcathinone The blood with a content of 50.42wt% is taken as blank blood, and a standard 4-methylmethcathinone is added. Add 25 mL of a mixed solution of toluene, anisole, dichloromethane and ether to sample one and sample two respectively, extract cathinone and 4-methyl methcathinone in the sample, toluene, anisole, dichloromethane The volume ratio of methane and ether is 6:3:2:1.5, add 20 mL of hydrochloric acid with a mass fraction of HCl of 20% to the separated organic phase, remove the organic phase, and concentrate the residue on a 35°C rapid concentrator to dryness , respectively add 20mL mobile phase extraction with a bottle-top pipette, shake for 10 minutes, and centrifuge for 5 minutes. Add 10 mL of mobile phase with a bottle-top pipe...
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