Preparation method and application of malignant tumour specific growth factor (TSGF) antigen electrogenerated chemiluminescence sensor

A technology for growth factors and malignant tumors, applied in the field of electrochemiluminescence detection, to achieve the effects of no radioactive pollution, wide signal response range, and high sensitivity

Inactive Publication Date: 2015-08-12
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a simple, rapid and pollution-free preparation method and application of nitrogen-doped carbon quantum dot electrochemiluminescence immun

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 A preparation method of an electrochemiluminescent sensor for malignant tumor-specific growth factor TSGF antigen:

[0028] 1. Preparation method of electrochemiluminescent sensor for malignant tumor-specific growth factor TSGF antigen

[0029] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0030] (2) Drop 6 μL, 8 μg / mL nitrogen-doped carbon quantum dot N-CQDs solution onto the electrode surface, rinse with ultrapure water, and dry at room temperature;

[0031] (3) When the electrode is almost dry, add 3 μL of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide EDC and 0.05 mol / L N- Hydroxysuccinimide NHS solution was applied to the surface of the electrode, incubated in a refrigerator at 4°C for 30 minutes, and dried at room temperature;

[0032] (4) Add 6 μL of bovine serum albumin BSA solution with a mass fraction of 0.5% on the surface of the electrode to seal t...

Embodiment 2

[0034] Example 2 Preparation method of an electrochemiluminescent sensor for malignant tumor-specific growth factor TSGF antigen

[0035] 1. Preparation method of electrochemiluminescent sensor for malignant tumor-specific growth factor TSGF antigen

[0036] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0037] (2) Drop 6 μL, 10 μg / mL nitrogen-doped carbon quantum dot N-CQDs solution onto the electrode surface, rinse with ultrapure water, and dry at room temperature;

[0038] (3) When the electrode is nearly dry, add 3 μL of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide EDC and 0.1 mol / L N- Hydroxysuccinimide NHS solution was applied to the surface of the electrode, incubated in a refrigerator at 4°C for 30 minutes, and dried at room temperature;

[0039](4) Add 6 μL bovine serum albumin BSA solution with a mass fraction of 1.0% dropwise on the electrode surface to seal the no...

Embodiment 3

[0041] Example 3 A method for preparing an electrochemiluminescent sensor for malignant tumor-specific growth factor TSGF antigen:

[0042] 1. Preparation method of electrochemiluminescent sensor for malignant tumor-specific growth factor TSGF antigen

[0043] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 Grinding with polishing powder, cleaning with ultrapure water;

[0044] (2) Drop 6 μL, 12 μg / mL nitrogen-doped carbon quantum dot N-CQDs solution onto the electrode surface, rinse with ultrapure water, and dry at room temperature;

[0045] (3) When the electrode is almost dry, add 3 μL of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide EDC and 0.2 mol / L N- Hydroxysuccinimide NHS solution was applied to the surface of the electrode, incubated in a refrigerator at 4°C for 30 minutes, and dried at room temperature;

[0046] (4) Add 6 μL of bovine serum albumin BSA solution with a mass fraction of 2.0% dropwise on the surface of the electrode t...

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Abstract

The invention relates to a preparation method and application of a malignant tumour specific growth factor (TSGF) antigen electrogenerated chemiluminescence sensor. The invention in particular relates to preparation and application of an electrogenerated chemiluminescence immunosensor taking aminated graphene GS-NH2 and an incubation substance of a detection antibody Ab2 as detection antibody labels and provides a simple, rapid and pollution-free preparation method and an application of a nitrogen doped carbon quantum dot electrogenerated chemiluminescence immunosensor. Linear range of the nitrogen doped carbon quantum dot electrogenerated chemiluminescence immunosensor is 0.01ng/mL-100ng/mL, and detection limit is 3.3pg/mL, so that rapid and sensitive detection on malignant TSGF antigen is realized.

Description

technical field [0001] The invention relates to a preparation method and application of an electrochemiluminescent sensor for malignant tumor specific growth factor TSGF antigen. Specifically related to a nitrogen-doped carbon quantum dot as a light-emitting material, aminated graphene GS-NH 2 with detection antibody Ab 2 The preparation and application of the incubation product as an electrochemiluminescence immunosensor for detecting antibody markers belongs to the technical field of electrochemiluminescence detection. Background technique [0002] As a new tumor marker, TSGF has broad spectrum, high sensitivity and high specificity in the diagnosis of lung cancer, and it has certain value in the auxiliary diagnosis of lung cancer. Tumor-specific growth factor (TSGF) is a factor that promotes the proliferation of tumors and surrounding capillaries and releases them into peripheral blood during the formation and growth of malignant tumors. It not only has the specificity ...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/58G01N21/76
CPCG01N21/76G01N33/57423G01N33/588G01N2800/7028
Inventor 周静魏琴于思琦马洪敏王晓东罗川南杜斌范大伟李贺吴丹
Owner UNIV OF JINAN
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