Apparatus and method for point-of-collection measurement of biomolecular reaction
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A technology of biomolecules and reactions, used in branch equipment, biological testing, chemical instruments and methods, etc.
Inactive Publication Date: 2015-08-12
CORNELL UNIVERSITY
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Most importantly, however, all of these existing smartphone-based systems rely on user input or physical measurements, which are often not pathologically specific
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Embodiment I
[0119] Example I: Quantitative Measurement of 25-Hydroxyvitamin D
[0120] In this example, using the Figure 7 A modular diagnostic testing platform ("test strip") 704 is shown. Included on the test strip 704 is a blood filter assembly 717 for filtering the raw blood sample into a plasma sample. The filter assembly 717 consists of a stack of reduced pore size commercial filters (Whatman, Kent, UK) ranging in pore size from approximately 2.7 μm (considering the approximate limit for filtering highly deformed red blood cells) to 0.7 μm (small enough to filter platelets). (It is worth noting that the pore size of these filters was not optimal for reproducibility, accuracy, and speed in this work).
[0121] The circulating concentration of a metabolite of vitamin D, 25-hydroxyvitamin D [25(OH)D], is considered the best indicator of vitamin D status. We have developed a nanoparticle-based assay and test strips that quantify 25(OH)D concentrations between 5 ng / ml and 75 ng / ml, ...
Embodiment II
[0126] Example II: Detection of Kaposi's Sarcoma-Associated Herpesvirus (KSHV) DNA
[0127] In this example, using image 3 A modular diagnostic test platform shown as 203, as described above.
[0128] BLAST primer design was used to design short DNA sequences for use as probes for KHSV DNA. Briefly, oligonucleotides were selected that were specific for KSHV DNA encoding vCyclin. Gold nanoparticles are attached to oligonucleotides via a 5' alkylthiol group. The nanoparticles have an average diameter of 15 nm, which is a compromise between the higher sensitivity of larger particles and the easier handling of the properties and stability of smaller particles. Add 100 μL of 100 μM KSHV to every 1 ml of 3 nM GNP solution, and react overnight. Then, concentrated solutions of sodium phosphate and sodium dodecyl sulfate (SDS) were added to make them respectively reach concentrations of 10 mM and 0.01% in the solution, and reacted overnight again. Next, sodium chloride was added ...
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Abstract
A system, methods, and apparatus for biomolecular measurements, monitoring, and tracking uses a smartphone-based system. A biological sample, including, but not limited to blood, saliva, biopsy, or sweat, is collected on a modular diagnostic test platform, which is then inserted into a smartphone accessory. Optical, electrical, mechanical, or other means are used to transduce a biomolecular binding event, including antibody, aptamer, enzymatic, base-pair matching, or other biological recognition reaction and communicate the results with the smartphone. Some specific examples of targets include 25-hydroxyvitamin D, folic acid, DNA, or proteins from infectious agents, and zinc. The result can then be presented quantitatively or turned into a more consumer-friendly measurement (positive, negative, above average, etc.), displayed to the user, stored for later comparison, and communicated to a central hub location where medical professionals can provide additional review. Additionally, social media integration can allow for device results to be broadcast to specific audiences, to compare healthy living with friends, to compete in health based games, create mappings, and other applications.
Description
[0001] related application [0002] This application claims priority to US Provisional Application Serial No. 61 / 699,486, filed September 11, 2012, the subject matter of which is incorporated herein by reference in its entirety. technical field [0003] Embodiments to which the present invention relates generally relate to biomolecular diagnostics, and more particularly, to portable point-of-collection diagnostic systems, methods and assemblies for biomolecular response measurement, monitoring, tracking and reporting, including social media applications. Background technique [0004] Modern endeavors in medicine and healthy living both involve individualized care and management of patients. Because of the high degree of variability inherent in biology, including diagnostic criteria, treatment, and disease management, often what is best for one patient may not be ideal for another. Gathering information on individuals is the first step before healthcare providers can provide...
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