Preparation method of moxidectin

A technology of moxidectin and bacterial strains, which is applied in the field of moxidectin preparation, can solve the problems of low output, high cost, and decreased drug properties, and achieve the effects of high output, simple production method, and simple fermentation process

Inactive Publication Date: 2015-08-19
WUHU FOMAN BIOPHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above-mentioned prior art, the purpose of the present invention is to overcome the high drug resistance of parasites to conventional anti-parasitic drugs in the prior art, so that the drug resistance is obviously reduced, and the production of new anti-pa

Method used

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preparation example Construction

[0016] The present invention provides a kind of preparation method of moxidectin, wherein, described preparation method comprises:

[0017] 1) Inoculate the strain in the strain culture medium and cultivate to obtain the spore suspension M1;

[0018] 2) inoculating the spore suspension M1 in the seed medium and culturing it to obtain the seed liquid M2;

[0019] 3) inoculating the seed solution M2 into a fermentation medium and culturing to obtain the initial extract M3;

[0020] 4) Moxidectin is prepared by subjecting the initial extract M3 to an upper protection reaction, an oxidation reaction, a deprotection reaction and an oximation reaction; wherein,

[0021] The strain is Streptomyces cyaneogriseus and / or Streptomyces hygroscopicus.

[0022] The above design prepares the spore suspension M1 by inoculating Streptomyces cyaneogriseus and / or Streptomyces hygroscopicus on the strain medium, and then inoculates the spore suspension M1 on the seed medium for cultivation, Th...

preparation example 1

[0037] Dissolve 3g of yeast powder, 10g of glucose, 3g of corn flour, 0.5g of sodium chloride, 0.05g of sodium molybdate, 0.05g of sodium acetate, 0.05g of potassium dihydrogen phosphate and 0.05g of dipotassium hydrogen phosphate in water, and then adjust the volume to 1000ml. Then place it in a pressure cooker at a temperature of 121° C. for 20 minutes to autoclave to prepare a culture medium for bacteria.

preparation example 2

[0039] Dissolve 5 g of glucose, 20 g of maltodextrin, 1 g of starch, 10 g of yeast extract, and 2 g of soybean cake powder in water, and then make the volume to 1000 ml, and then put it in a pressure cooker at 121°C for 20 minutes to autoclave to prepare the seed medium.

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Abstract

The invention discloses a preparation method of moxidectin. The preparation method comprises the following steps: 1) inoculating a strain in a strain culture medium for culture to obtain spore suspension M1; 2) inoculating the spore suspension M1 in a seed culture medium for culture to obtain seed solution M2; 3) inoculating the seed solution M2 in a fermentation culture medium for culture to obtain primary extract solution M3; 4) performing protection reaction, oxidation reaction, deprotection reaction and oximation reaction to the primary extract solution M3 to obtain the moxidectin, wherein the strain is Streptomyces cyaneogriseus and/or Streptomyces hygroscopicus. By adopting the method, the total yield of the prepared moxidectin is enabled to be obviously higher and thus the effects of simpler and more convenient production manner and higher yield are realized.

Description

technical field [0001] The invention relates to the field of production and preparation of moxidectin, in particular to a preparation method of moxidectin. Background technique [0002] Moxidectin is a new type of antiparasitic drug, a single-component macrolide antibiotic fermented by Streptomyces. As an antiparasitic drug, it is widely used in basic agriculture such as animal husbandry and aquaculture, and with the development of agriculture and other fields, the international demand for antiparasitic drugs has continued to grow. At present, the annual market demand for this product is about 10 tons, and the market size exceeds 200 million US dollars. [0003] From the 1970s to the 1980s, my country had synthetically produced drugs such as hexachloro-p-xylene, nitrothiocyanamide, thiobisdichlorophene, and niclosamide, which effectively prevented and controlled livestock parasitic diseases that commonly occurred in my country. Since the 1990s, my country has carried out r...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12R1/55C12R1/465
Inventor 苏福男
Owner WUHU FOMAN BIOPHARMA CO LTD
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