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A method of using yeast cell wall to co-produce glucan and mannoprotein

A yeast cell wall and mannoprotein technology, applied in fermentation and other directions, can solve the problems of waste of raw materials, increase explosion-proof measures, increase environmental protection pressure, etc., and achieve the effects of high product safety, shortening production time, and reducing emissions

Active Publication Date: 2017-12-22
珠海天香苑生物科技发展股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although, at present, yeast cell walls are used as raw materials to produce yeast glucan and mannoprotein (Peter Phillips CN101087872B, Yu Xuefeng CN102051400B, Tanaka Mei and CN101018852A), but these methods are all to extract glucan or mannoprotein separately, Most of these methods are characterized by destructive methods to remove another component to obtain a single product, which not only wastes raw materials, but also is not conducive to environmental protection.
Although there are also a few patents that can extract the active substances in two or three kinds of yeast cell walls at one time (Yu Xuefeng CN101570769A, Chen Xiaochun, etc. CN102329380B), these methods have used strong alkali (sodium hydroxide or potassium hydroxide), organic solvent (n-butyl Alcohol, chloroform, etc.), while dextran and mannoprotein are mainly used in food. These organic solvents have brought certain safety hazards to the product, and at the same time increased the safety restrictions during production (production equipment must be strictly ventilated, and explosion-proof Measures), a large number of lye used increased environmental pressure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 100kg of yeast cell wall, add water to make the volume to 5% mass volume ratio; 60Mpa high-pressure homogeneous spraying treatment 6 times; adjust pH to 9.0-11.0, adopt the method of continuous steam elimination, pass the cell wall solution through a steam injector, and steam tubes to maintain 120-135°C, continuous elimination for 60s; cool down to 50°C, add 3% alkaline protease (based on yeast cell wall), and keep warm for 18h.

[0026] Centrifuge. The centrifuged heavy liquid was washed with an equal volume of water, repeated 8 times, and spray-dried to obtain 20.0 kg of dextran. The centrifuged light liquid was filtered through an alkali-resistant nanofiltration membrane (molecular weight cut-off 200Da). Continue to add 6 times the volume of water to wash and filter. The retentate continues to be filtered with an ultrafiltration membrane with a molecular weight of 10000-30000Da, and when the permeate of the ultrafiltration membrane is concentrated to a concent...

Embodiment 2

[0028] Take 100kg of yeast cell wall, add water to make the volume to 10% mass volume ratio; 60Mpa high-pressure homogeneous spraying treatment 6 times; adjust pH to 9.0-11.0, adopt the method of continuous steam elimination, pass the cell wall solution through the steam injector, and the steam tube maintains 120-135°C, continuous elimination for 120s; cool down to 55°C, add 2% alkaline protease (based on yeast cell wall), and keep warm for 12h.

[0029] Centrifuge. The centrifuged heavy liquid was washed with an equal volume of water, repeated 8 times, and spray-dried to obtain 18.2 kg of dextran. The centrifuged light liquid was filtered through an alkali-resistant nanofiltration membrane (molecular weight cut-off 200Da). Continue to add 6 times the volume of water to wash and filter. The retentate continues to be filtered with an ultrafiltration membrane with a molecular weight of 10000-30000Da, and when the permeate of the ultrafiltration membrane is concentrated to a co...

Embodiment 3

[0031] Take 100kg of yeast cell wall, add water to make the volume to 8% mass volume ratio; 40Mpa high-pressure homogeneous spraying treatment 3 times; adjust pH to 9.0-11.0, adopt the method of continuous steam elimination, pass the cell wall solution through a steam injector, and steam tubes to maintain 120-135°C, continuous elimination for 90s; cool down to 50°C, add 1% alkaline protease (based on yeast cell wall), and keep warm for 15h.

[0032] Centrifuge. The centrifuged heavy liquid was washed with an equal volume of water, repeated 8 times, and spray-dried to obtain 16.4 kg of dextran. The centrifuged light liquid was filtered through an alkali-resistant nanofiltration membrane (molecular weight cut-off 200Da). Continue to add 6 times the volume of water to wash and filter. The retentate continues to be filtered with an ultrafiltration membrane with a molecular weight of 10000-30000 Da, and when the permeate of the ultrafiltration membrane is concentrated to a concen...

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PUM

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Abstract

The invention provides a method for co-production of glucan and mannose glycoprotein in use of yeast cell walls. The method comprises the steps of: taking yeast cell walls and adding water; carrying out high-pressure homogenization treatment; regulating pH and adopting a steam continuous-elimination method; cooling, and adding alkaline protease to carry out enzymolysis; carrying out centrifugal separation, adding water to centrifugal heavy liquid to wash, and drying to obtain glucan; filtering centrifugal light liquid with an alkali-resisting nanofiltration membrane, concentrating and drying trapped liquid to obtain mannose glycoprotein. According to the method for co-production of glucan and mannose glycoprotein in use of yeast cell walls, the glucan produced from the yeast cell walls with low additional value as the raw material can be applied to foods and plays good auxiliary treatment role in patients with diabetes, obesity, constipation and high cholesterol; the glucan and the mannose glycoprotein are extracted at a time, so that production time is shortened; moreover, yields of the glucan and the mannose glycoprotein are high; no organic solvent is used in the production process and the products have relatively high safety; no strong alkali treatment is used in the production process, so that emission of pollutants is reduced and the method is very important to the environmental protection.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for co-producing glucan and mannoprotein by using yeast cell walls. Background technique [0002] Our country is very rich in yeast resources, and the yeast deep processing industry is also relatively developed. The annual output of yeast extract is about 60,000 tons, which is accompanied by the production of a large number of yeast cell walls. Yeast cell walls are generally used as feed products, and the added value of the product is not high. [0003] Yeast β-glucan has a unique triple helix structure, and there are some hydrophilic groups on the outside of the helix, which can specifically bind to the body's immune cell receptors and stimulate them to be in a highly active state, which can stimulate humoral immunity and cellular immunity , has non-specific immune mechanism and hematopoietic function, and can play anti-tumor, scavenging free radicals, anti-oxid...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/08C12P21/06
Inventor 陈远才陈雪松熊明洲张敬松周志刚
Owner 珠海天香苑生物科技发展股份有限公司