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In-situ preparation-based method for detecting concentration of glucose by use of fluorescent Ag nano-clusters

A technology of glucose concentration and silver nanoclusters, which is applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve problems such as unavoidable false positive signals, and achieve the effect of avoiding interference and excellent fluorescence properties

Active Publication Date: 2015-08-26
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, although it has been reported that fluorescent metal nanoclusters can be used to detect glucose (Nano Lett., 2013, 13, 309-314; Biosens. Bioelectron., 2011, 26, 1965-1969; Anal. Chim. Acta., 2012, 749,56-62), but these methods are all realized by utilizing the fluorescence quenching effect of hydrogen peroxide, the product of glucose catalytic oxidation, on nanoclusters. This fluorescence "turn-off" method may be difficult to avoid false positive signals during detection. interference

Method used

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  • In-situ preparation-based method for detecting concentration of glucose by use of fluorescent Ag nano-clusters
  • In-situ preparation-based method for detecting concentration of glucose by use of fluorescent Ag nano-clusters
  • In-situ preparation-based method for detecting concentration of glucose by use of fluorescent Ag nano-clusters

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Different concentrations (225, 300, 400, 500, 600, 700, 800, 900, 1000, 1100 and 1200 μM) of D-glucose were mixed with GOx at a final concentration of 5 μg / mL and a pH value of 5.5, and the final concentration was 5 μg / mL. A certain volume of water was added to the citric acid buffer solution with a concentration of 5 mM to make the total volume 400 μL, and the samples were incubated in a 37° C. water bath for 2 h. Add MAA at a final concentration of 400 mM and FeSO at a final concentration of 1 mM 4 With a pH value of 3.0, a final concentration of 10mM citric acid buffer solution and an appropriate amount of deionized water, the total volume after mixing was 500μL, left at room temperature for 30min, and dialyzed for 12h with a dialysis bag with a molecular weight of 1000Da. Take out the above 200 μL solution and add AgNO with a final concentration of 20 mM 3 , HAc-NaAc buffer solution with a pH value of 4.5, a final concentration of 10mM, and an appropriate amount of...

Embodiment 2

[0022] Configure a glucose solution with a concentration of 550 μM as an unknown concentration glucose sample to test the accuracy of the detection method. The steps are the same as in Example 1. We tested four sets of data, and their fluorescence emission intensities were 200.5, 219.1, 205.4, and 201.9 respectively, and from the standard curve The calculated standard value of fluorescence emission intensity was 212.1. Compared with the standard value, the relative errors of each detection of glucose concentration were 5.5%, 3.3%, 3.2%, 4.8%, and the average error was 4.2%.

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Abstract

An in-situ preparation-based method for detecting the concentration of glucose by use of fluorescent Ag nano-clusters belongs to the technical field of fluorescent Ag nano-clusters. In the presence of O2, glucose oxidase (GOx) catalyzes and oxidizes glucose to generate H2O2, a free radical produced by 'Fenton Reaction' between H2O2 and Fe<2+> is used for arousing methacrylic acid (MAA) polymerization, the polymerization product polymethyl acrylic acid (PMAA) serves as a stabilizer to prepare fluorescent Ag nano-clusters (Ag NCs) through ultraviolet light reduction method, and the fluorescent emission intensity of the acquired nano-clusters and the concentration of glucose are utilized to build a quantitative relationship, so that the glucose concentration detection can be realized. The prepared Ag NCs is uniform in particle diameter and less than 2 nm in size and has excellent orange fluorescent emission. The method for glucose detection is a fluorescent 'turn-on' process, and the optimal glucose detection condition can be obtained through optimization of the preparation condition.

Description

technical field [0001] The invention belongs to the technical field of application of fluorescent silver nanoclusters, in particular to a method for detecting glucose concentration based on in situ prepared fluorescent silver nanoclusters. Background technique [0002] Metal nanoclusters are a new type of material whose size is close to the Fermi wavelength of electrons and between metal atoms and nanoparticles. Due to the ultra-small size of nanoclusters, they have strong fluorescence emission, good stability and It has good biocompatibility and other advantages, so it has high application value in the field of small biological molecule detection. [0003] Glucose is an important carbohydrate in organisms. It is an essential nutrient for human metabolism, an energy source for cells and an intermediate product of metabolism. Abnormal glucose concentration can usually cause serious diseases, such as diabetes, etc. Therefore, glucose detection is very important in life scienc...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 林权陈阳于聪孙源卿陈洁杨雪杨柏董凤霞
Owner JILIN UNIV
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