In-situ preparation-based method for detecting concentration of glucose by use of fluorescent Ag nano-clusters
A technology of glucose concentration and silver nanoclusters, which is applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve problems such as unavoidable false positive signals, and achieve the effect of avoiding interference and excellent fluorescence properties
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[0019] Example 1
[0020] Different concentrations (225, 300, 400, 500, 600, 700, 800, 900, 1000, 1100 and 1200 μM) of D-glucose were mixed with GOx at a final concentration of 5 μg / mL and pH 5.5, respectively. A certain volume of water was added to a citric acid buffer solution with a concentration of 5 mM to make the total volume 400 μL, and the samples were incubated in a 37° C. water bath for 2 h. Add MAA at a final concentration of 400 mM and FeSO at a final concentration of 1 mM 4 and citric acid buffer solution with a pH value of 3.0 and a final concentration of 10 mM and an appropriate amount of deionized water, the total volume after mixing was 500 μL, placed at room temperature for 30 min, and dialyzed with a dialysis bag with a molecular weight of 1000 Da for 12 h. Take out 200 μL of the above solution and add AgNO with a final concentration of 20 mM 3 , pH 4.5, HAc-NaAc buffer solution with a final concentration of 10 mM and an appropriate amount of water to make...
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[0021] Example 2
[0022] A glucose solution with a concentration of 550 μM was configured as a glucose sample of unknown concentration to test the accuracy of the detection method. The steps were the same as those in Example 1. We tested four sets of data, and their fluorescence emission intensities were 200.5, 219.1, 205.4, and 201.9, respectively. The calculated standard value of fluorescence emission intensity was 212.1. Compared with the standard value, the relative error of each detected glucose concentration was 5.5%, 3.3%, 3.2%, and 4.8%, and the average error was 4.2%.
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