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Soybean molasses hypsizigus marmoreus liquid nutrient medium and application thereof

A liquid culture medium, soybean molasses technology, applied in the direction of microorganism-based methods, microorganisms, fungi, etc., to achieve the effect of improving the market application scope, improving the quality, and reducing the diameter of the fungus ball

Inactive Publication Date: 2015-09-02
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soybean molasses has a large reserve in the market and is cheap. It is widely used in the process of adding animal feed, but it has not been reported as an additive in the preparation of liquid strains of Japonica japonica

Method used

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  • Soybean molasses hypsizigus marmoreus liquid nutrient medium and application thereof
  • Soybean molasses hypsizigus marmoreus liquid nutrient medium and application thereof
  • Soybean molasses hypsizigus marmoreus liquid nutrient medium and application thereof

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Embodiment 1

[0022] The composition content of the medium is: 200g of potatoes, 10g of glucose, 3g of peptone, 1g of yeast powder, 1.5g of potassium dihydrogen phosphate 7 water, 1.5g of magnesium sulfate, 10mL of soybean molasses, add water to 1L, and the pH is 5.3. The culture medium was sterilized at 115° C. for 30 minutes, and then used after cooling.

[0023] (1) Use single distilled water to prepare liquid medium according to the recipe, take half of the liquid medium and add soybean molasses with a final concentration of 1% (molasses / total volume) according to the concentration, and then use 4M NaOH aqueous solution to adjust the pH to the same as the control group (no added molasses) consistent;

[0024] (2) Divide the prepared liquid culture medium into 250ml shake flasks, each bottle is divided into 100ml, 15 bottles are prepared for a total of 30 bottles, sterilized at 115°C for 30 minutes, and cooled for later use;

[0025] (3) Take the activated agaricus plate strains, use a ...

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Abstract

The invention relates to a soybean molasses hypsizigus marmoreus liquid nutrient medium and application thereof and provides the recipe of the liquid nutrient medium which can promote hypsizigus marmoreus mycelial growth and increase pellet number. The soybean molasses hypsizigus marmoreus liquid nutrient medium has the advantages that when the liquid nutrient medium is used to cultivate hypsizigus marmoreus, the mycelial concentration of the hypsizigus marmoreus can reach the maximum value of about 10g / L on the sixth day, pellet diameter can reach 0.1-0.5mm, the mycelial concentration is 3-4 times of that of a control group, and the pellet diameter of the control group is 1-3mm; the hypsizigus marmoreus metabolic capability is increased evidently; soybean molasses serving as cheap materials are widely applied to animal feed, but no reports indicate that the soybean molasses are used as additives during fungus liquid spawn production.

Description

technical field [0001] The invention belongs to the field of biological fermentation engineering, and in particular relates to the preparation of the formula of the liquid culture medium for the application of soybean molasses in the preparation of the liquid strain of the jaundice mushroom. Background technique [0002] Banyu mushroom, also known as jiji mushroom and crab-flavored mushroom, belongs to Basidiomvcotina, Hvmenomvcetes, Agaicales, white mushroom family, jade mushroom ( Hypsizigus ), known as "Royal Mushroom" or "Royal Mushroom" in Japan, is a rare edible fungus that is favored by domestic and foreign markets. Guangdong and other provinces (cities) have promoted cultivation, and a certain scale has been formed, and the output continues to rise. [0003] The japonica mushroom factory generally uses solid inoculation in the inoculation of fungus bags. Solid inoculation is usually divided into two methods: branch inoculation and bacterial block inoculation. There ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 胡开辉林辉孙淑静傅俊生
Owner FUJIAN AGRI & FORESTRY UNIV
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