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Astaxanthin esterase production strain and application of strain in preparation of free astaxanthin

A technology of astaxanthin ester and astaxanthin, which is applied to astaxanthin esterase production strains and its application field in the preparation of free astaxanthin, can solve the problems of multiple by-products, waste, difficult products, etc., and achieve high efficiency The effect of preparation

Active Publication Date: 2015-09-02
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of hydrolyzing astaxanthin mainly adopts saponification. Although this method has the advantage of rapid reaction, the reaction process is too violent and it is difficult to control the product. High concentration of alkali will also damage astaxanthin and produce more side effects. Products, such as producing astaxanthin and half astaxanthin, etc.
Not only that, the reaction process uses a large amount of alkali and organic solvents, which not only damages the environment, but also causes great waste, which is not in line with the national development strategy
The other is to use lipase enzymatic hydrolysis to prepare free astaxanthin by using lipase to degrade astaxanthin ester, which provides a new idea for the production of free astaxanthin, but the enzymatic hydrolysis process is complicated and requires Hydrolyze triglycerides in advance and remove free fatty acids, then use lipase to hydrolyze astaxanthin esters, which is time-consuming and expensive
Moreover, due to the water insolubility and complex composition of astaxanthin ester, the enzymatic hydrolysis efficiency of lipase is low, which limits its large-scale preparation process.
It is considered an effective method to prepare free astaxanthin by fermentation, but the current mixed fermentation method cannot realize the complete hydrolysis of astaxanthin esters, and the industrialization process of free astaxanthin production is still unrealized

Method used

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Embodiment Construction

[0022] The present invention will be described in detail below in conjunction with the examples.

[0023] 1. Screening of strains degrading astaxanthin ester

[0024] The medium formula used in the present invention is as follows:

[0025] 1) Enrichment medium

[0026] NH 4 NO 3 0.1%, KH 2 PO 4 0.025%, MgSO 4 ·7H 2 O 0.025%, FeSO 4 ·7H 2 O 0.001%, cholesterol oleate 0.1%, tap water, pH 7.0, sterilized at 115°C for 30min.

[0027] 2) Screening plate solid medium (solid screening plate)

[0028] NH 4 NO 3 0.1%, KH 2 PO 4 0.025%, MgSO 4 ·7H 2 O 0.025%, FeSO 4 ·7H 2 O 0.001%, cholesterol oleate (1.0% Triton X-100 solubilization) 0.1%, agar 2%, tap water, pH 7.0, sterilized at 115°C for 30min.

[0029] 3) Fermentation medium

[0030] Haematococcus pluvialis oil fermentation medium: KH 2 PO 4 0.025%, MgSO 4 ·7H 2 O 0.025%, FeSO 4 ·7H 2 O 0.001%, beef extract 0.1%, peptone 1%, Haematococcus pluvialis oil (0.4% Tween80 solubilizing) 0.5%, tap water, pH 7...

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Abstract

The invention aims at providing an astaxanthin esterase production strain and application of the astaxanthin esterase production strain in preparation of free astaxanthin. The strain for producing lipase and esterase is stenotrophomonas and stored with the number of CGMCC NO.10672. The stenotrophomonas strain can be used for producing free astaxanthin by efficiently utilizing an organic solvent extract of haematococcus pluvialis oil and / or aquatic animal (such as shrimp and crab); the strain can effectively degrade astaxanthin ester to prepare free astaxanthin; in addition, the experiment shows that the quantity of added tween 80 in fermenting liquid, inoculation amount, initial pH of fermenting liquid and fementing time condition can be determined; a method for efficiently preparing the free astaxanthin is provided.

Description

technical field [0001] The invention belongs to the technical field of functional microorganism screening, and in particular relates to an astaxanthin esterase production strain and its application in the preparation of free astaxanthin. Background technique [0002] Astaxanthin (also known as 3,3'-dihydroxy-4,4'-diketone-β,β-carotene) is an unsaturated terpene compound, a kind of carotenoid, which exists widely In organisms, especially aquatic organisms such as shrimp, crab, algae and fish. Astaxanthin is purple-red, easy to oxidize, and becomes astaxanthin (Astacene) after oxidation. Astaxanthin is insoluble in water, has fat solubility, and is easily soluble in chloroform, acetone, benzene and carbon disulfide. [0003] A large number of studies have found that astaxanthin has good biological functions, such as anti-aging, anti-oxidation, enhancing the body's immunity, and anti-tumor. Astaxanthin's unique molecular structure makes it the strongest antioxidant in nature...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P23/00C12R1/01
CPCC12N1/20C12P23/00C12N1/205C12R2001/01
Inventor 毛相朝董浩薛长湖
Owner OCEAN UNIV OF CHINA
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