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Plant extract as well as preparation process and application thereof

A technology of plant extract and preparation process, which is applied in the field of plant extract and its preparation for pest control, to achieve the effect of no secondary pollution, simple preparation process and high insecticidal activity

Active Publication Date: 2015-09-09
湖南一科生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no literature report that palmetto leaves or their extracts have the biological activity of killing aphids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Preparation of palm leaf alcohol extract

[0019] 0.5kg of dried palmetto leaves were crushed into fine powder and ultrasonically extracted three times with absolute ethanol, the ratio of solid to liquid was 1:10, 1:5 and 1:5 (w / v) respectively, and each extraction was 2h. The ultrasonic frequency is 26.4kHz, the water bath ensures the extraction temperature is 50°C, and the stirring speed is 27rpm. Filtrate, combine the extracts obtained three times, centrifuge at 2000rpm, and concentrate the supernatant by rotary evaporation at a temperature of 45°C and a vacuum of 0.1MPa until there is no smell of ethanol, and 41.66g of dry extract is obtained with a yield of 8.3%, which is palm leaf Alcoholic extract.

Embodiment 2

[0022] Example 2 Insecticidal Activity Test of Palmflower Leaf Extract on Aphids

[0023] Weigh 1g of the palm leaf alcohol extract prepared in Example 1, dissolve it with 4mL of 80% ethanol, and then make it to 10mL with sterile water. At this time, the mass concentration of the medicinal solution is 100g / L. 100mL, to get 1g / L drug solution, add 2-3 drops of Tween-80 to the diluent to form a uniform and stable liquid. In the same way, the palm leaf water extract prepared in Comparative Example 1 was formulated into a 1 g / L medicinal solution; the control was 80% ethanol 4 mL, and was diluted to 10 mL with sterile water, and then diluted 100 times with sterile water.

[0024] Gather radish aphids from the field ( Lipaphis erysimi Kaltenbach ), put them on small rapeseed indoors, and when the population multiplies to a sufficient number, collect rapeseed leaves with aphids, use a brush to remove nymphs and winged aphids, and make the aphids basically the same size and moder...

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PUM

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Abstract

The invention discloses plant extract as well as a preparation process and application thereof. The plant extract is livistona chinensis leaf alcohol extract. The preparation process comprises the following steps: crushing livistona chinensis leaves; adding absolute ethyl alcohol and carrying out ultrasonic digestion, wherein the material-to-liquid ratio is 1 to (3-15) (w / v); centrifuging an obtained extracted solution; separating liquid supernatant; and rotating, evaporating and concentrating until no alcohol taste exists to obtain the livistona chinensis leaf alcohol extract. Effective components are extracted from the livistona chinensis leaves to be used as active components of a pesticide, so that the livistona chinensis leaf alcohol extract for effectively killing aphids is provided, and a novel thought is provided for disinsection and pest control in agricultural production.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and in particular relates to a plant extract used for exterminating insects and pests, as well as its preparation process and application. Background technique [0002] Aphids [Myzus persicae (Sulzer), Aphis gossypii Glover, Brevicorynebrassicae (L.), Lipaphis erysimi (Kaltenbach), etc.], spider mites [Tetranychuscinnabarinus (Boiduval), T. . truncates Ehara and two-spotted spider mite T. urticae Koch, etc.] are the main pests that harm melon and vegetable crops. With the global warming and the continuous expansion of the cultivated area in protected areas, the damage caused by them is serious year by year. First of all, high temperature can increase the number of occurrence bases and increase the number of occurrence generations, and the protected area becomes a place for a large number of pests to overwinter and reproduce in winter. Secondly, in protected cultivation, the natural en...

Claims

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Application Information

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IPC IPC(8): A01N65/40A01P7/04
Inventor 杜杭华
Owner 湖南一科生物科技有限公司
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