Bacteria removal method during the cultivation of Chlamydomonas reinhardtii by using mixed antibacterial agents
The technology of an antibacterial agent and rhinoceros is applied in the field of removing bacteria and fungi from Chlamydomonas reinhardtii, which can solve problems such as contamination of miscellaneous bacteria, and achieve the effects of strong killing ability, convenient preparation, and prevention and control of contamination by miscellaneous bacteria.
Active Publication Date: 2018-01-19
XUZHOU NORMAL UNIVERSITY
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[0007] The purpose of the present invention is to provide a method for removing bacteria in the cultivation process of Chlamydomonas reinhardtii by using mixed antibacterial agents, so as to solve the problem of bacterial contamination in the experimental and application process of Chlamydomonas control
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Embodiment 1
[0026] Embodiment 1: A. Inoculate the Chlamydomonas mixed bacteria contamination on the TAP solid medium plate, at 23 ± 0.5 ° C, 14 / 10 hours light and dark photoperiod, 8000Lx light intensity to cultivate for 3-5 days; B. Prepare a mixed antibacterial agent TAP solid medium plate containing 10ug / ml azoxystrobin and 15ug / ml nalidixic acid for use; On the TAP plate, culture at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity for 5-8 days; D. Transfer the bacterium-eliminated Chlamydomonas to ordinary TAP solid again On the medium plate, culture at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity for 3-5 days, for subsequent experiments or preservation for future use.
[0027] The specific steps are:
[0028] 1. Activation of contaminated Chlamydomonas: inoculate the contaminated Chlamydomonas on a TAP solid medium plate, and cultivate it for 3-5 days at 23±0.5°C, 14 / 10 hours light and dark photoperiod, and 8000Lx light inten...
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The invention relates to a method for removing bacteria in the cultivation process of Chlamydomonas reinhardtii by using mixed antibacterial agents, which belongs to the method for removing bacteria and fungi of Chlamydomonas reinhardtii. Method: A. Inoculate Chlamydomonas contaminated by mixed bacteria on a TAP solid medium plate, and cultivate it for 3-5 days at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity; B. Prepare 10ug / The mixed antibacterial agent TAP solid medium plate of ml azoxystrobin and 15ug / ml nalidixic acid is ready for use; C. Transfer the cultured Chlamydomonas containing miscellaneous bacteria to the TAP plate containing the mixed antibacterial agent , cultured at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity for 5-8 days; D. Transfer the Chlamydomonas that has been sterilized to the common TAP solid medium plate again, Cultivate for 3-5 days at 23±0.5°C, 14 / 10 hour light and dark photoperiod, and 8000Lx light intensity, for subsequent experiments or preservation for future use. The advantage is that azoxystrobin is used to remove fungi, nalidixic acid is used to remove bacteria, and the problem of miscellaneous bacteria pollution generated during scientific experiments and applications using Chlamydomonas reinhardtii is solved.
Description
technical field [0001] The invention relates to a method for eliminating bacteria and fungi of Chlamydomonas reinhardtii, in particular to a method for eliminating bacteria in the cultivation process of Chlamydomonas reinhardtii by using mixed antibacterial agents. Background technique [0002] Chlamydomonas reinhardtii is a eukaryotic unicellular microalgae with short growth cycle, easy cultivation, annotated genome, mature related genetic biochemical technology and molecular biology technology, heavy metal adsorption capacity, high oil production, etc. As a model organism for the research and application of life sciences, the research on Chlamydomonas, a model of ciliated diseases, and the research on biodiesel and biodecontamination have been carried out. However, in the process of scientific research and application, it is inevitable to cause the pollution of Chlamydomonas species during inoculation, and these miscellaneous bacteria pollution will endanger the preservati...
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IPC IPC(8): C12N1/12A01N43/90A01P1/00A01P3/00A01N43/54
Inventor 王亮陆军郑元林陈慧怡杨丰源
Owner XUZHOU NORMAL UNIVERSITY