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Method for screening silicate bacteria from manganese nodule

A technology of silicate bacteria and manganese nodules, applied in the field of screening, isolation and cultivation of silicate microbial strains, achieving high efficiency, promoting work efficiency and reliable results

Inactive Publication Date: 2015-09-09
INST OF PROCESS ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the invention is to solve the problem of screening silicate bacteria

Method used

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  • Method for screening silicate bacteria from manganese nodule
  • Method for screening silicate bacteria from manganese nodule
  • Method for screening silicate bacteria from manganese nodule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] see figure 1 , Weigh 10 g of oceanic manganese nodule sample, put it in a conical flask, add 90 mL of sterilized distilled water, add glass beads, and shake it in a shaker to obtain a sample bacterial suspension. The obtained sample bacterial suspension was diluted step by step and spread on a plate, and then cultured upside down in a constant temperature incubator. After 24 h, the growth state of the colony was observed. Pick a typical silicate single colony and continue to culture after streaking on the medium. Repeat this operation for 4 times with the obtained bacteria, and it can be seen that the shape and size of the bacteria are basically the same under the microscope. Bacteria are selected again for plate culture, sent to the testing unit for testing the gene sequence, and the results are obtained. The reference species of the obtained bacterium is Azotobacter chroococcum (Azotobacter genus), belonging to the genus Bacteria; Proteobacteria; Gammaproteobacte...

Embodiment 2

[0031] see figure 1 , Weigh 10 g of oceanic manganese nodule sample, put it in a conical flask, add 90 mL of sterilized distilled water, add glass beads, and shake it in a shaker to obtain a sample bacterial suspension. The obtained sample bacterial suspension was diluted step by step and spread on a plate, and then cultured upside down in a constant temperature incubator. After 24 h, the growth state of the colony was observed. Pick a typical silicate single colony and continue to culture after streaking on the medium. Repeat this operation for 4 times with the obtained bacteria, and it can be seen that the shape and size of the bacteria are basically the same under the microscope. Bacteria are selected again for plate culture, and sent to the detection unit for testing the gene sequence, and the results are obtained. The reference species of this bacterium is Azotobacter chroococcum (Azotobacter rotundum), which belongs to Bacteria; Proteobacteria; Gammaproteobacteria; ...

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Abstract

The invention provides a method for screening silicate bacteria from a manganese nodule. The method comprises the following steps that a manganese nodule sample is weighed, added into sterilized distilled water and scattered evenly, and the turbid liquid with the concentration of 0.05-0.2 g / mL is obtained; the obtained turbid liquid is diluted step by step and sprayed on a plate, and the plate coated with turbid liquid is reversely cultivated in a constant-temperature cultivation box; a typical bacterial colony is selected from obtained bacterial colonies and placed on a culture medium for lineation and continuous cultivation, and the typical bacterial colony is a transparent or semitransparent and bacterial colony semi-glass-bead protruding bacterial colony which is not prone to being lifted or being drawn after being lifted; the culture medium is a nitrogen-free solid selective culture medium; the processing process of the steps is repeatedly executed till the bacterial colony is purified. The method has the advantages of being high in efficiency, easy to operate, reliable in result and the like, and the working efficiency of screening microorganisms from minerals can be improved.

Description

technical field [0001] The invention relates to a screening method for microbial strains, in particular to a screening, isolation and culturing method for silicate microbial strains in oceanic manganese nodule samples. Background technique [0002] Microorganisms, especially those in extreme environments like the ocean, play an extremely important role in natural biology and geochemical cycles. The study of microorganisms in the ocean will help us understand and understand the origin and evolution of life, as well as the tolerance mechanism of microorganisms and their interaction with the environment in the ocean environment. The competition for microbial strain resources, especially those with special value, has become an important part of today's international competition for biological resources. [0003] Silicate bacteria refer to bacteria that can decompose silicate minerals, mainly including bacillus circulans and bacillus mucilaginosus. Alexandrov of the former Sovi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/065
CPCC12N1/20
Inventor 李浩然滕青
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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