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A method of detecting the content of triacontanol in a foliar fertilizer by applying gas chromatography

A technology of triacontanol and foliar fertilizers, which is applied in the directions of measuring devices, material separation, and material analysis, can solve problems such as bud sheath bending, young leaves curling, and root deformity at the seedling stage, and achieve fast processing and high content Detect accurate effects

Active Publication Date: 2015-09-09
SHANDONG INST FOR PROD QUALITY INSPECTION
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

However, if triacontanol is used excessively, it will cause the sprouts to bend at the seedling stage, the roots will be deformed, and the young leaves will be curled when the adult plant is grown. Moreover, it is forbidden to add or excessively add plant fertilizers to new fertilizers that are mainly foliar fertilizers The situation of growth regulators occurs from time to time, therefore, it is very important to monitor the quality of triacontanol preparations

Method used

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  • A method of detecting the content of triacontanol in a foliar fertilizer by applying gas chromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Accurately pipette 1.0g of liquid foliar fertilizer, dissolve it with 30mL of chloroform, add 5mL of phosphoric acid to it, ultrasonicate for 30min, shake for 30min, then rest for 30min, take out the lower organic phase, and continue to extract the upper emulsion with 30mL of chloroform Twice, combine the chloroform phase, rotary evaporate to near dryness, set the volume to 25mL, take 1mL to pass through a 0.22μm filter membrane, to be tested;

[0025] The above liquid to be tested was analyzed by a gas chromatograph. The gas chromatograph conditions were: HP-5 capillary column, 30mm×320μm×0.25μm; inlet temperature: 280°C, splitless; detector temperature: 320°C; Column temperature: adopt temperature program, initial temperature is 160°C, keep for 1min, increase to 320°C at 15°C / min, keep for 3min; carrier gas is nitrogen: flow rate is 1.0mL / min; injection volume: 1μL; use external standard method Obtain the concentration of triacontanol in the liquid to be tested, and t...

Embodiment 2

[0027] Accurately pipette 1.0g of powder foliar fertilizer, dissolve it with 30mL of chloroform, add 5mL of phosphoric acid to it, sonicate for 30min, shake for 30min, rest for 30min, centrifuge at 8000rpm for 5min, remove the precipitate, add the supernatant to 20mL of ultrapure water in a separatory funnel, shake intermittently for 5 minutes, and stand still for 30 minutes. After the chloroform phase and the water phase are separated, remove the chloroform phase, rotary evaporate to near dryness, set the volume to 25mL, and take 1mL to pass through 0.22μm filter membrane, to be tested;

[0028] The above liquid to be tested was analyzed by a gas chromatograph. The gas chromatograph conditions were: HP-5 capillary column, 30mm×320μm×0.25μm; inlet temperature: 300°C, splitless; detector temperature: 330°C; Column temperature: adopt temperature program, the initial temperature is 200°C, keep for 1min, rise to 320°C at 15°C / min, keep for 3min; carrier gas is nitrogen: flow rate ...

Embodiment 3

[0030] Accurately pipette 1.0g of crushed solid foliage fertilizer, dissolve it with 30mL of chloroform, add 5mL of phosphoric acid to it, ultrasonicate for 30min, shake for 30min, rest for 30min, centrifuge at 8000rpm for 5min, remove the precipitate, add the supernatant to 20mL In ultrapure water, transfer to a separatory funnel, shake intermittently for 5 minutes, and let stand for 30 minutes. After the chloroform phase and the water phase are separated, remove the chloroform phase, rotary evaporate to nearly dryness, set the volume to 25mL, and take 1mL Pass through a 0.22μm filter membrane, to be tested;

[0031] The above-mentioned liquid to be tested is analyzed by a gas chromatograph, and the conditions of the gas chromatograph are: the conditions of the gas chromatograph include HP-5 capillary chromatographic column, 30mm×320μm×0.25μm; the temperature of the inlet: 300°C, splitless; the detection Detector temperature: 320°C; column temperature: adopt temperature progr...

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Abstract

The invention relates to a detecting method of triacontanol, and particularly relates to a method of detecting the content of triacontanol in a foliar fertilizer by applying gas chromatography. The method includes weighing a triacontanol standard substance, dissolving into trichloromethane to prepare a series of triacontanol solutions having known concentrations, subjecting the triacontanol solutions to gas chromatography analysis to obtain triacontanol peak areas corresponding to the series of concentrations, acquiring a standard curve and an equation of linear regression thereof by adopting the triacontanol concentrations as horizontal coordinates and by adopting the peak areas as vertical coordinates, processing a sample to be detected, subjecting the sample to gas chromatography analysis to obtain the peak area of the triacontanol in the sample, and substituting the peak area into the equation of linear regression to obtain the corresponding triacontanol concentration. The method is rapid in processing process and accurate in content detection, and can answer to quality monitoring of illegal triacontanol addition or excessive triacontanol addition in the market.

Description

(1) Technical field [0001] The invention relates to a method for detecting triacontanol, in particular to a method for detecting triacontanol content in foliar fertilizers by gas chromatography. (2) Background technology [0002] Triacontanol (1-Triacontanol) is a long-chain fatty alcohol composed of 30 carbon atoms, also known as meliflora alcohol. In 1977, research by Rics and others proved that triacontanol is a new plant growth stimulant, which has various physiological functions such as promoting rooting, germination, flowering, stem and leaf growth, and early maturity, as well as increasing chlorophyll content and enhancing photosynthesis. , It can also promote the absorption of nitrogen and phosphorus fertilizers by rice seedlings and cucumber seedlings. Triacontanol always coexists with other long-chain alcohols in nature. Experiments have proved that only triacontanol has physiological activity, while other long-chain alkanes not only do not promote plant growth, b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 张娟邹惠玲刘爽许士明王东岳朱海荣杜伯会范晓明周莉莉王景超夏攀登
Owner SHANDONG INST FOR PROD QUALITY INSPECTION
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