A kind of preparation method of diesel oil degrading bacteria immobilized particle

Abstract

Provided is a preparation method of diesel degradable bacterium immobilized particles. The preparation method mainly comprises the steps that an artemia cyst eggshell carrier is dried, then ground and sterilized under high pressure; a strain Arthrobacter cultured through an LB culture medium and the artemia cyst eggshell sterilized under high pressure are mixed and are subjected to adsorption culture for 4-10 hours on the conditions of 100-130 rpm / min and 25-37 DEG C; after adsorption, centrifugation is performed on the aseptic condition of 6,000-12,000 rpm / min for 2-10 min; an upper clear liquid is abandoned; the artemia cyst eggshell carrier is added into a sodium alginate solution to be mixed evenly; a pipette gun is used for adding the sodium alginate solution into a calcium chloride solution dropwise, crosslinking is performed for 20-50 min, and immobilized particles of 3-5 mm are obtained; unwrapped strains on the surfaces of the immobilized particles are flushed away through sterile double distilled water, and then the diesel degradable bacterium immobilized particles are obtained and stored at 4 DEG C. The preparation method is easy to operate and free of secondary pollution, and degradability of diesel by the prepared immobilized particles can reach 65%.

Description

technical field [0001] The invention belongs to the field of microorganisms and environmental engineering, in particular to a preparation method of a diesel degradation material. Background technique [0002] In recent years, with the wide application of petroleum and petroleum products, the leakage of petroleum mining, smelting and transportation, and the discharge of oily wastewater have caused serious environmental pollution. Commonly used methods to control oil pollution include physical methods, chemical methods and bioremediation methods. Physical methods are suitable for recovering sudden oil spills and controlling the spread of oil spills, but the treatment efficiency is greatly affected by weather, ocean conditions, and types of oil spills; chemical methods may cause secondary pollution, complex production processes, and high costs. It is difficult to be applied in practice; chemical combustion produces black smoke, causes secondary pollution, and wastes energy. ...

AI Technical Summary

Problems solved by technology

At present, traditional biological methods such as: activated sludge method (sBR method), biofilm method (membrane bioreactor), contact oxidation method and sequential batch biological treatment are mostly used; among them, the activated sludge method has a better treatment effect , but has higher requirements for water inflow; compared with the activated sludge method, the biofilm method is attached to the surface of the filler carrier, so that microorganisms with slow reproduction speed can also exist, thus forming a stable ecosystem. However, the microbial The amount is difficult to control, so it is not easy to operate

[0004] Although the traditional biological method has its unique advantages, because the microorganisms usually grow in suspension in water, the concentration of degrading bacteria is low, the bacteria are easy to lose, the competitiveness with the environment is weak, and the ability to resist toxic pollution is poor.

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