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Solid culture medium for inoculating liquid samples and culture method

A solid culture medium and solid culture technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of cumbersome operation, poor timeliness, and long time, and achieve good transparency, controllable shape, and water absorption. big effect

Inactive Publication Date: 2015-09-16
汇征联合(北京)医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, the present invention solves the problems in the prior art that a large number of liquid specimens are first cultured in liquid and then cultured in solid, and the whole process takes a long time, the operation is cumbersome, and the timeliness is poor, and then provides a method applicable to a large amount of liquid Solid medium and culture method for direct culture of specimens, easy separation, counting and observation, strong timeliness, and simple operation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Prepare agar medium according to the conventional method, pour it into a plate, and use it as an agar medium reinforcement layer, pour 1ml of liquid sample into the agar medium reinforcement layer, and then evenly cover a layer of 1mm konjac gum dry powder as a liquid specimen adsorption layer ;

[0035] (2) Put the inoculated culture medium surface up, put it into a 37° C. incubator, and cultivate it for 18 hours, and then perform further detection.

[0036] White konjac gum dry powder becomes a transparent colloid after absorbing water, which is convenient for observing the growth of bacterial colonies, counting colonies or picking a single colony for further testing.

[0037] The water-absorbing powder layer of the liquid specimen adsorption layer in this embodiment can also be chitosan, carboxymethyl cellulose, gelatin, xanthan gum, guar gum, carrageenan, polyethylene glycol with a degree of deacetylation of more than 90%. One or several combinations of diols, ...

Embodiment 2

[0039](1) Dissolving carboxymethyl cellulose in water to make a 0.5wt% solution, autoclaving, laying a film with a thickness of 1mm, and preparing a liquid specimen adsorption layer for future use.

[0040] (2) To prepare the agar medium reinforcement layer, pour 12ml of liquid sample into the agar medium reinforcement layer, then cover the above-mentioned liquid sample adsorption layer, with the surface of the medium upward, put it into a 37°C incubator, cultivate for 18 hours, and then for further testing

[0041] In this embodiment, the liquid sample adsorption layer can also be one or more combinations of chitosan, agarose, gelatin, xanthan gum, guar gum, and polyethylene glycol with a degree of deacetylation of more than 90%. The material becomes transparent after absorbing water and is stable to temperature.

Embodiment 3

[0043] (1) Prepare a solution of 0.45wt% agar, 8wt% gelatin, and 0.6wt% konjac gum, add 6wt% nutrients needed for microbial growth, autoclave, inject a flat plate, and have a thickness of 4mm to prepare a liquid specimen adsorption layer.

[0044] (2) Pour 5ml of the liquid sample into the liquid sample adsorption layer, with the surface of the culture medium facing up, put it into a 37°C incubator, and incubate for 18 hours before further detection.

[0045] In this embodiment, one or more of microbial growth regulators, hemolytic agents, lysing agents, antibiotic neutralizers, and bacterial selective growth agents can also be added to promote microbial growth or remove substances that may affect microbial growth in liquid samples. substance.

[0046] The above-mentioned liquid specimen adsorption layer, the gel matrix made of superabsorbent material has a pore size small enough to filter or intercept bacteria on the surface of the solid medium, and fully absorb excess liquid...

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Abstract

The invention discloses a solid culture medium for inoculating a great amount of liquid samples and a culture method. The solid culture medium is provided with a culture layer, wherein the culture layer at least comprises a liquid sample adsorption layer; the liquid sample adsorption layer is a water absorption membrane layer and / or a water absorption powder layer. The solid culture medium can absorb liquid in the liquid sample, a great amount of liquid samples can be directly inoculated and cultured, and then counting, observation, separation or identification is further carried out on the solid culture medium according to a purpose. The solid culture method for a great amount of liquid sample microorganisms is characterized in that a great amount of liquid samples are poured into the solid culture medium to be subjected to solid culture, the solid culture does not need to be followed by the liquid culture, the culture and separation can be integrated according to the requirement, the microorganisms can be quantified while being cultured, a bacterial colony can be separated, the positive rate of the cultured microorganisms can be increased, the culture procedure can be simplified, the culture time can be saved, and the timeliness can be improved.

Description

technical field [0001] The invention relates to a solid culture medium for inoculating a large amount of liquid specimens and a culture method, belonging to the technical field of microorganism culture. Background technique [0002] Microbial culture methods are divided into solid culture methods and liquid culture methods due to different culture media; wherein, the commonly used culture media are solid culture media and liquid culture media. Solid medium is a solid form, and microorganisms grow on the surface of the medium. It is widely used in the isolation, identification, preservation, counting and observation of colony characteristics of microorganisms. The liquid culture gene nutrients are evenly distributed, fully in contact with the surface of microorganisms, can dissolve a large number of microbial metabolites, and can inoculate a large number of specimens, and the positive rate of cultured microorganisms is high; however, liquid culture medium is not easy to quant...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
Inventor 唐明忠
Owner 汇征联合(北京)医疗器械有限公司
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