Siraitia grosvenorii tissue culture seedling propagation method

A technology of tissue culture seedlings and Luo Han Guo, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of seedlings that cannot be planted in large quantities, virus accumulation, and variety degradation, etc., and achieve high yield, fast reproduction, The effect of less disease

Inactive Publication Date: 2015-09-23
GUANGXI UNIV
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Problems solved by technology

However, the traditional planting of Mangosteen is planted with seed potatoes. The disadvantages of seed potato plan...

Method used

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Embodiment Construction

[0034] A kind of Luo Han Guo tissue culture seedling breeding method, this breeding method comprises the following steps:

[0035] (1) Collection of propagation materials:

[0036] Choose to collect branches from varieties and individual plants that grow robustly, come to flowers in a timely manner, are easy to bear fruit, and have large and neat fruit;

[0037] (2) Disinfection treatment:

[0038] Cut young shoots of about 50 cm, rinse them with sterile water on the workbench of the tissue culture room, immerse them in 75% alcohol solution for 5 seconds, then immerse them in 0.1% mercuric solution for 10 minutes, take them out and rinse them with sterile water ;

[0039] (3) Virus-free cultivation of shoot tips:

[0040] Prepare the basic medium with MS+BA1.0mg / L+IAA0.1mg / L+agar 0.35%+sucrose 3%, adjust the pH value to 6, divide the prepared medium, each bottle is about 2cm high, Tighten the cap tightly, let it cool naturally after autoclaving;

[0041] Cutting out the s...

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Abstract

The invention discloses a siraitia grosvenorii tissue culture seedling propagation method, which comprises the following steps of (1) collection of a propagation material, (2) disinfection treatment, (3) virus-free culture of stem tips, (4) secondary culture of segments, (5) induced rooting culture, (6) transplantation, (7) seedling raising in a greenhouse and (8) discharging from the greenhouse. According to the siraitia grosvenorii tissue culture seedling propagation method, tissue culture seedlings are cultured by a cutting technology under an aseptic condition, tissue culture seedling planting has the characteristics of high yield, less plant disease, high propagation speed, high adaptability, capability of planting and harvesting in the same year and high yield, and conventional seed potato planting is gradually replaced by tissue culture seedling planting.

Description

technical field [0001] The invention relates to the technical field of fruit tree cultivation, in particular to a method for growing Luo Han Guo tissue cultured seedlings. Background technique [0002] The cultivation of Luo Han Guo has a long history in my country. In recent years, studies have found that the use value of Luo Han Guo is very high. People are more and more optimistic about the market prospect of Luo Han Guo, and the planting scale continues to expand. However, the traditional cultivation of Luo Han Guo is planted with seed potatoes. The disadvantages of seed potato planting are low yield, more virus accumulation, variety degradation, and the seedlings cannot meet the needs of mass planting. In recent years, people have carried out in-depth research on a series of technologies such as cultivating improved varieties of Luo Han Guo, standardized planting and demonstration, especially in the rapid propagation and detoxification of fine varieties through tissue c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 农守枫
Owner GUANGXI UNIV
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