Kit for detecting child active tuberculosis and application of kit

A tuberculosis and active technology, which is applied in the field of kits for detecting active tuberculosis in children, can solve the problems of low diagnostic accuracy, inability to obtain, poor tracheal ciliary motility, etc., achieve high sensitivity and specificity, simple method, Design reasonable and feasible effects

Inactive Publication Date: 2015-09-23
BEIJING CHILDRENS HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, for a long time, the diagnosis of tuberculosis in children has faced two practical problems that are difficult to solve: first, the bacterial load of tuberculosis patients in children is low, coupled with poor cough reflex and tracheal ciliary motility in children, often accompanied by extrapulmonary tuberculosis, etiological diagnosis Positive rate is very low
When the clinical symptoms of children with tuberculosis are not obvious in the early stage, timely and accurate etiological results cannot be obtained, and the diagnosis is often delayed, resulting in the deterioration of the disease and the loss of the best time for rescue
2. When the immune function is low, or the child suffers from sever

Method used

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  • Kit for detecting child active tuberculosis and application of kit
  • Kit for detecting child active tuberculosis and application of kit
  • Kit for detecting child active tuberculosis and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, the detection method of active tuberculosis in children

[0054] 1. Sample collection and preparation

[0055] 1. The source of the sample

[0056] Plasma samples were selected from 42 cases (from Beijing Children’s Hospital Affiliated to Capital Medical University), of which 24 cases were from children with active tuberculosis and the other 18 were from healthy children. The children with active tuberculosis were all confirmed by clinical diagnosis and informed consent was obtained . All plasma samples were drawn in the morning on an empty stomach, and the plasma was separated and stored in a -80°C low-temperature refrigerator for later use.

[0057] 2. Sample preparation

[0058] (1) Plasma collection: collect venous blood in BD tubes to avoid hemolysis. Slowly shake the tube up and down five times to mix the clot in the blood. Blood clots at room temperature (25°C) for 1 hour and placed vertically. Centrifuge SST tubes (vacuum blood collection tub...

Embodiment 2

[0081] Embodiment 2, the application of the method for detecting active tuberculosis in children

[0082] A total of 42 plasma samples were selected, 22 of which were used for model training, including 11 children with active tuberculosis and 11 healthy children; the remaining 20 samples were used for model testing, including 7 healthy children and 13 children with active tuberculosis. Children with active tuberculosis were confirmed by clinical diagnosis. All plasma samples were drawn in the morning on an empty stomach, and the plasma was separated and stored in a -80 low-temperature refrigerator.

[0083] 1. Train 22 samples (11 children with active tuberculosis and 11 healthy children) with the method for detecting active tuberculosis in children in Example 1.

[0084] The results show that the correct rate of this method is 100%. The random selection method is used for cross-validation, and the verification result is 100%, which shows that the method has good predictive a...

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Abstract

The invention discloses a kit for detecting child active tuberculosis and an application of the kit. The kit is combined with a bioinformatics method to screen characteristic metabolic products of child active tuberculosis, and a detecting method for the child active tuberculosis is established based on chemical shift of the characteristic metabolic products of child active tuberculosis; experiments show that in verification of 24 samples by the detecting method provided by the invention, the accuracy is 100%, the specificity is 100% and the sensitivity is 100%; in addition, the detecting method is simple and easy to operate and can be used for early detection of child active tuberculosis; the detecting method provides a new approach for clinical diagnosis of child active tuberculosis as well as provides new thoughts for exploring disease mechanisms.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a kit for detecting active tuberculosis in children and its application. Background technique [0002] Tuberculosis caused by Mycobacterium tuberculosis (MTB) is one of the most common human infectious diseases in the world today and is considered to be the second largest disease "killer" after AIDS. Therefore, tuberculosis has become a threat to human health. The most important disease is a difficult problem of global public health control. China is one of the 22 countries with a high incidence of tuberculosis in the world, and the number of tuberculosis patients ranks second in the world. The epidemic situation of tuberculosis among children in my country is also severe. The fourth national tuberculosis epidemiological sampling survey report in 2000 showed that the infection rate of tuberculosis among children aged 0-14 in my country was 9.0%, and the prevalence of ac...

Claims

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Application Information

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IPC IPC(8): G01N24/08
Inventor 申阿东孙琳李洁琼徐放肖婧焦伟伟綦辉申晨
Owner BEIJING CHILDRENS HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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