Ultrporous nanofiber mats and uses thereof

A nanofiber and fiber technology, applied in the field of liquid filtration media, can solve problems that have not been reported yet, achieve high water flux and reduce the amount of time

Active Publication Date: 2015-09-23
MILLIPORE CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Specifically, the pads have not been reported to indicate any ability of currently known virus retention membranes

Method used

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  • Ultrporous nanofiber mats and uses thereof
  • Ultrporous nanofiber mats and uses thereof
  • Ultrporous nanofiber mats and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] Example 1. Preparation of Nylon stock solution for electrospinning

[0112] This example provides an exemplary procedure for preparing a nylon solution for electrospinning according to an embodiment of the invention.

[0113] Nylon 6 is supplied by BASF Corporation (Florham Park, NJ, USA) under the trademark Ultramid B24. A 15% wt. solution was prepared from a mixture of three solvents (formic acid, acetic acid and water) present in a weight ratio of 2:2:1. The solutions were prepared by vigorously stirring a mixture of solvent and polymer at 80°C for 5 to 6 hours in a glass reactor. It was then cooled to room temperature. The molecular weight of the final polymer was analyzed by SEC and found to decrease due to heating in this solvent system (Table 1). In addition, the molecular weight distribution (Mw / Mn) also decreased.

[0114] Table 1. Molecular weight analysis of nylon 6 before preparation of stock solutions and as a result of hydrolysis.

[0115]

[0116]...

Embodiment 2

[0117] Example 2. Preparation of Nylon Solutions Ready for Electrospinning According to Embodiments of the Invention.

[0118] The stock solution prepared in Example 1 was diluted to 8% wt. polymer with 2,2,2-trifluoroethanol (TFE), formic acid and water. Ammonium formate was added to a concentration of 1% wt. The composition of the spinning solution is listed in Table 2.

[0119] Table 2. Composition of the spinning solution.

[0120]

[0121] The viscosity of the final solution at 25°C was 30 cP to 35 cP, and the conductivity was 1.0 mS / cm to 1.5 mS / cm.

Embodiment 3

[0122] Example 3. Preparation of Parvovirus Retention Pads According to Embodiments of the Invention.

[0123] Nanospider purchased from Elmarco (Morrisville, NC USA) was used immediately TM The solution from Example 2 was spun by a nozzleless electrospinning device. The 6-wire rotating electrode is equipped with 33-gauge steel wire, the distance between the solution pan (pan) and the collector is 140mm, the electrode rotation speed is 60Hz, and the humidity is maintained between 10° dew point and 16° dew point using an external humidification system . The spinning time was 30 min. In this embodiment, the support material used to collect the nanofibers was also an electrospun nylon 6 mat with an average fiber diameter of about 100 nm. In this embodiment, the supportive electrospun material was prepared from a 12 wt. Nylon 6 is produced by electrospinning, and the weight ratio between the acids is 2:1.

[0124] Table 3 summarizes the properties of the fabricated electrospu...

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Abstract

A porous electrospun polymeric nanofiber liquid filtration medium, such as an electrospun mats, used for the removal of viral particles (e.g., parvovirus) and other particles in the 18 nm to 30 nm size range from fluid streams, having a mean flow bubble point measured with perfluorohexane above 100 psi. The electrospun medium includes nanofibers having an average fiber diameter of about 6 nm to about 13 nm, and the nanofiber liquid filtration medium has a mean pore size ranging from about 0,01 um to about 0,03 um, a porosity ranging from about 80% to about 95%, a thickness ranging from about 1 um to about 100 um, and a liquid permeability greater than about 10 LMH / psi. The high porosity of the electrospun mats enable much higher water fluxes, thus reducing the time required to complete virus filtration steps on a fluid stream.

Description

[0001] Description of the invention technical field [0002] The present invention generally relates to liquid filtration media. In certain embodiments, the present invention provides methods of preparing nanofibrous polymeric materials with extremely small fiber diameters, methods of assembling these fibers into highly consistent mats, and methods of using the mats to remove viruses from fluid streams. Background of the invention [0003] Regulatory agencies around the world strictly require commercial manufacturers of biopharmaceutical compounds to provide assurance of the biosafety of their drugs. Manufacturers must insert and validate at least two orthogonal (operating by two different mechanisms) steps of virus removal in their process, one of which is usually size-based filtration. The estimated LRV (log reduction value) of the filtration is at least 4. [0004] Current virus filtration strategies are provided in Meltzer, T. and Jornitz, M. eds., "Filtration and Puri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D39/14B01D39/08B01D69/06
CPCB01D2323/39B01D71/56B01D69/06B01D39/14B01D39/08B01D67/0004B01D61/145B01D39/1623B01D2239/025B01D2239/0631B01D2239/0654B01D2239/1233A61L2/022B01D2325/0281B01D2325/02832
Inventor M·科兹洛夫G·特卡奇克O·Y·科斯A·波蒂J·帕特尔
Owner MILLIPORE CORP
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