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Preparing method of matrine nanoparticles modified by wheat germ agglutinin and application of matrine nanoparticles

A technology of wheat germ agglutinin and matrine, which is applied in the field of medicine, can solve the problems of low targeting and poor application effect of matrine, and achieve tumor-specific targeting, low cost, and easy preparation conditions Effect

Inactive Publication Date: 2015-09-30
HENAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above situation, in order to solve the defects of the prior art, the object of the present invention is to provide a preparation method of matrine nanoparticles modified by wheat germ agglutinin and its application, which can effectively solve the problem of matrine nanoparticles modified by wheat germ agglutinin. The preparation of granules and the problem of low targeting of matrine and poor use effect

Method used

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  • Preparing method of matrine nanoparticles modified by wheat germ agglutinin and application of matrine nanoparticles
  • Preparing method of matrine nanoparticles modified by wheat germ agglutinin and application of matrine nanoparticles
  • Preparing method of matrine nanoparticles modified by wheat germ agglutinin and application of matrine nanoparticles

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Embodiment 1

[0016] The preparation method of matrine nanoparticles modified by wheat germ agglutinin of the present invention comprises the following steps:

[0017] (1) Preparation of matrine nanoparticles (Mt-NPs): Weigh 0.5g of matrine (MT), heat and dissolve in 1.0ml water bath to form matrine aqueous solution; weigh polylactic acid-glycolic acid copolymer (PLGA ) 0.279g, heated and dissolved in 20ml acetone water bath, as the organic phase; at 1600r min -1 Under stirring at a rotating speed, the matrine aqueous solution is dripped into the organic phase to form colostrum, and 125 ml of an aqueous solution of polyvinyl alcohol (PVA) with a mass concentration of 0.46% is used as the water phase, and the mixture is heated at 815r·min. -1 Under stirring at a rotating speed, inject the colostrum into the water phase, continue to stir for 2 hours, evaporate the acetone, and put the -1 Centrifuge for 1 hour, collect the precipitate, wash the precipitate with distilled water three times, pr...

Embodiment 2

[0020] A preparation method of matrine nanoparticles modified by wheat germ agglutinin, comprising the following steps:

[0021] (1) Preparation of matrine nanoparticles (Mt-NPs): Weigh 0.4g of matrine (MT), heat and dissolve in a 0.8ml water bath to form matrine aqueous solution; weigh polylactic acid-glycolic acid copolymer (PLGA ) 0.26g, heated and dissolved in 18ml acetone water bath, as the organic phase; at 1600r·min -1 Under stirring at a rotating speed, the matrine aqueous solution is dripped into the organic phase to form colostrum, and 122 ml of an aqueous solution of polyvinyl alcohol (PVA) with a mass concentration of 0.46% is used as the water phase, and the mixture is heated at 815 r min -1 Under stirring at a rotating speed, inject the colostrum into the water phase, continue to stir for 2 hours, evaporate the acetone, and put the -1 Centrifuge for 1 h, collect the precipitate, wash the precipitate with distilled water three times, pre-freeze at -50°C for 10 h,...

Embodiment 3

[0024] A preparation method of matrine nanoparticles modified by wheat germ agglutinin, comprising the following steps:

[0025] (1) Preparation of matrine nanoparticles (Mt-NPs): Weigh 0.6g of matrine (MT), heat and dissolve in 1.2ml water bath to form matrine aqueous solution; weigh polylactic acid-glycolic acid copolymer (PLGA ) 0.29g, heated and dissolved in 21ml acetone water bath, as the organic phase; at 1600r·min -1 Under rotating speed stirring, the matrine aqueous solution is dripped in the organic phase, forms colostrum, is the polyvinyl alcohol (PVA) aqueous solution 128ml of 0.46% with mass concentration as water phase, at 815r·min -1 Under stirring at a rotating speed, inject the colostrum into the water phase, continue to stir for 2 hours, evaporate the acetone, and put the -1 Centrifuge for 1 hour, collect the precipitate, wash the precipitate with distilled water three times, pre-freeze at -50°C for 10 hours, raise the temperature to -30°C and maintain it for...

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Abstract

The invention relates to a preparing method of matrine nanoparticles modified by wheat germ agglutinin and application of the matrine nanoparticles. The method and application effectively solve the problems that preparing of the matrine nanoparticles modified by the wheat germ agglutinin and matrine are low in targeting and poor in using effect. According to the matrine nanoparticles modified by the wheat germ agglutinin, the matrine nanoparticles are connected with wheat germ agglutinin through chemical bonds, and matrine is dissolved into a water solution through water bath; polylactic acid-hydroxyacetic acid copolymer acetone is dissolved into an organic phase through water bath; the matrine water solution is dripped into the organic phase to form colostrums, the colostrums is injected into a polyving akohol water solution, acetone is volatilized, centrifugation is conducted, sediment and drying are conducted, and matrine nanoparticles are obtained; the matrine nanoparticles are dissolved through a PBS solution, carbodiimide and N-hydroxy succinimide is added, activation and centrifugation are conducted, supernate is added into a solution containing wheat germ agglutinin, incubation and centrifugation are conducted, sediment and drying are conducted, and the final matrine nanoparticles is prepared. According to the preparing method, the preparation condition is met easily, the raw material source is rich, the cost is low, and the preparing method is innovation of cancer therapeutic drug.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a preparation method and application of matrine nanoparticles modified by wheat germ agglutinin. Background technique [0002] Colon cancer is one of the common malignant tumors of the digestive tract, ranking second in the cause of death of cancer patients in European and American countries, and gradually showing a high incidence trend in my country. How to make anticancer drugs into nanoparticles and endow them with the function of actively recognizing cancer cells is a common concern in the field of pharmacy. Chunxia Wang et al. used wheat germ agglutinin to modify paclitaxel nanoparticles to study its effect on the proliferation of colon cancer cells CaCo-2 and HT-29. Compared with ordinary paclitaxel nanoparticles, the results showed that glycoproteins on colon cancer cells had an effect on wheat germ. Lectins were expressed at higher levels than in normal colonocytes. Ying Liu et ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/14A61K31/4375A61K47/42A61K47/34A61P35/00
Inventor 段晓颖程远方刘丽娜桑峰康冰亚王丽萍崔琳刘真
Owner HENAN UNIV OF CHINESE MEDICINE
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